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Plant and Cell Physiology 2004 45(8):1099-1104; doi:10.1093/pcp/pch117
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© 2004 Oxford University Press

Short Communication

Stable Isotope Labeling of Arabidopsis thaliana for an NMR-Based Metabolomics Approach

Jun Kikuchi1,2, Kazuo Shinozaki3,4 and Takashi Hirayama2,3,4,5

1 Protein Research Group, Genomic Sciences Center, RIKEN Yokohama Institute, 1-7-22 Suehiro, Tsurumi, Yokohama, 230-0045 Japan
2 Graduate School of Integrated Science, Yokohama City University, 1-7-29 Suehiro, Tsurumi, Yokohama, 230-0045 Japan
3 Plant Functional Genomics Research Group, Genomic Sciences Center, RIKEN Yokohama Institute, 1-7-22 Suehiro, Tsurumi, Yokohama, 230-0045 Japan
4 Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074 Japan

Abstract

Nuclear magnetic resonance (NMR) will become a key technology in plant metabolomics with the use of stable isotope labeling and advanced hetero-nuclear NMR methodologies. To demonstrate the power of this approach, we performed multi-dimensional hetero-nuclear NMR analysis of metabolic movement of carbon and nitrogen nuclei in Arabidopsis thaliana. First, distinct ethanol-stress response was investigated using 13C-labeled wild type and an ethanol-hypersensitive mutant plants. Furthermore, we followed nitrogen fluxes in 15N-labeled seeds during the initiation of germination in vivo. The future role of stable isotope-labeling combined with advanced hetero-nuclear NMR in plant metabolomics is discussed.

Footnotes

5 Corresponding author: E-mail: hirayama{at}gsc.riken.jp: Fax: +81-45-508-7363.


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