Skip Navigation

Plant and Cell Physiology 2004 45(7):887-896; doi:10.1093/pcp/pch097
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (26)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Chaerle, L.
Right arrow Articles by Van Der Straeten, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chaerle, L.
Right arrow Articles by Van Der Straeten, D.
Agricola
Right arrow Articles by Chaerle, L.
Right arrow Articles by Van Der Straeten, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2004 Oxford University Press

Thermal and Chlorophyll-Fluorescence Imaging Distinguish Plant-Pathogen Interactions at an Early Stage

Laury Chaerle1, Dik Hagenbeek1, Erik De Bruyne2, Roland Valcke3 and Dominique Van Der Straeten1,4

1 Department of Molecular Genetics, Unit Hormone Signalling and Bio-imaging, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium
2 Advanta SES-Europe, Biotechnology Department, Soldatenplein Z2, Industriepark 15, B-3300 Tienen, Belgium
3 Laboratory for Molecular and Physical Plant Physiology, Limburgs Universitair Centrum, Department SBG, Universitaire Campus, B-3590 Diepenbeek, Belgium

Different biotic stresses yield specific symptoms, owing to their distinct influence on a plant’s physiological status. To monitor early changes in a plant’s physiological status upon pathogen attack, chlorophyll fluorescence imaging (Chl-FI) and thermography, which respectively visualize photosynthetic efficiency and transpiration, were carried out in parallel for two fundamentally different plant–pathogen interactions. These non-destructive imaging techniques were able to visualize infections at an early stage, before damage appeared. Under growth-room conditions, a robotized set-up captured time series of visual, thermal and chlorophyll fluorescence images from infected regions on attached leaves. As a first symptom of the plant–virus interaction between resistant tobacco and tobacco mosaic virus (TMV), thermal imaging detected a local rise in temperature while Chl-FI monitored a co-localized increase in fluorescence intensity. Chl-FI also revealed pre-symptomatic high-intensity spots for the plant–fungus system sugar beet–Cercospora beticola. Concomitantly, spots of lower temperature were monitored with thermography, in marked contrast with our observations on TMV-infection in tobacco. Knowledge of disease signatures for different plant–pathogen interactions could allow early identification of emerging biotic stresses in crops, facilitating the containment of disease outbreaks. Presymptomatic monitoring clearly opens perspectives for quantitative screening for disease resistance, either on excised leaf pieces or attached leaves.

4 Corresponding author: Email, dominique.vanderstraeten{at}ugent.be; Fax, +32-9-264-5333.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
P. Muhlenbock, M. Plaszczyca, M. Plaszczyca, E. Mellerowicz, and S. Karpinski
Lysigenous Aerenchyma Formation in Arabidopsis Is Controlled by LESION SIMULATING DISEASE1
PLANT CELL, November 1, 2007; 19(11): 3819 - 3830.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Lenk, L. Chaerle, E. E. Pfundel, G. Langsdorf, D. Hagenbeek, H. K. Lichtenthaler, D. Van Der Straeten, and C. Buschmann
Multispectral fluorescence and reflectance imaging at the leaf level and its possible applications
J. Exp. Bot., March 1, 2007; 58(4): 807 - 814.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Berger, Z. Benediktyova, K. Matous, K. Bonfig, M. J. Mueller, L. Nedbal, and T. Roitsch
Visualization of dynamics of plant-pathogen interaction by novel combination of chlorophyll fluorescence imaging and statistical analysis: differential effects of virulent and avirulent strains of P. syringae and of oxylipins on A. thaliana
J. Exp. Bot., March 1, 2007; 58(4): 797 - 806.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. Gielen, M. Low, G. Deckmyn, U. Metzger, F. Franck, C. Heerdt, R. Matyssek, R. Valcke, and R. Ceulemans
Chronic ozone exposure affects leaf senescence of adult beech trees: a chlorophyll fluorescence approach
J. Exp. Bot., March 1, 2007; 58(4): 785 - 795.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Chaerle, I. Leinonen, H. G. Jones, and D. Van Der Straeten
Monitoring and screening plant populations with combined thermal and chlorophyll fluorescence imaging
J. Exp. Bot., March 1, 2007; 58(4): 773 - 784.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
L. Chaerle, M. Pineda, R. Romero-Aranda, D. Van Der Straeten, and M. Baron
Robotized Thermal and Chlorophyll Fluorescence Imaging of Pepper Mild Mottle Virus Infection in Nicotiana benthamiana
Plant Cell Physiol., September 1, 2006; 47(9): 1323 - 1336.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E-C Oerke, U Steiner, H-W Dehne, and M Lindenthal
Thermal imaging of cucumber leaves affected by downy mildew and environmental conditions
J. Exp. Bot., June 1, 2006; 57(9): 2121 - 2132.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.