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Plant and Cell Physiology 2004 45(7):864-871; doi:10.1093/pcp/pch104
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© 2004 Oxford University Press

The Accumulation of {alpha}-Zein in Transgenic Tobacco Endosperm is Stabilized by Co-expression of ß-Zein

Craig E. Coleman1,3, Paula Randall Yoho1, Sonja Escobar1 and Masahiro Ogawa2

1 Department of Plant and Animal Sciences, Brigham Young University, Provo, UT 84602, U.S.A.
2 Department of Life Science, Yamaguchi Prefectural University, 3-2-1 Sakurabatake, Yamaguchi City, Yamaguchi, 753 Japan

The cysteine-poor {alpha}-zein is the major prolamin storage protein fraction in maize endosperm and is localized in the interior of protein bodies with {delta}-zein, whereas the hydrophobic cysteine-rich ß- and {gamma}-zein are found on the exterior of the PB. In transgenic tobacco endosperm expressing zein genes, {alpha}-zein was unstable unless co-expressed with {gamma}-zein. Here we showed that {alpha}-zein was also stabilized by ß-zein. Small accretions of {alpha}- and ß-zeins, similar in appearance to maize protein bodies, were localized to the endoplasmic reticulum within tobacco endosperm cells. The zein proteins were also localized to protein storage vacuoles in a more dispersed pattern, suggesting that they were transported there after they were post-translationally sequestered into the ER.

3 Corresponding author: E-mail, craig_coleman{at}byu.edu; Fax, +1-801-422-0008.


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