Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Yoneda, A.
Right arrow Articles by Hasezawa, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoneda, A.
Right arrow Articles by Hasezawa, S.
Agricola
Right arrow Articles by Yoneda, A.
Right arrow Articles by Hasezawa, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2004, Vol. 45, No. 6 761-769
© 2004 Oxford University Press

Disruption of Actin Microfilaments Causes Cortical Microtubule Disorganization and Extra-Phragmoplast Formation at M/G1 Interface in Synchronized Tobacco Cells

Arata Yoneda, Minori Akatsuka, Fumi Kumagai and Seiichiro Hasezawa1

Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa, Chiba, 277-8562 Japan

The roles of actin microfilaments (MFs) in the organization of microtubules (MTs) at the M/G1 interface were investigated in transgenic tobacco BY-2 cells stably expressing a GFP-tubulin fusion protein, using the MF-disrupting agent, Bistheonellide A (BA). When MFs were disrupted by BA treatment, cortical MTs (CMTs) did not become reorganized even 3 h after phragmoplast collapse, whereas non-treated cells completed CMT reorganization within 1 h. Furthermore, in the absence of MFs, the tubulin proteins did not show appropriate recruitment but remained at the site where the phragmoplast had existed, or extra-phragmoplasts were organized. These extra-phragmoplasts could functionally form extra-cell plates. This is the first observation of the formation of multiple cell plates during one nuclear division, and of phragmoplast generation irrespective of the position of the mitotic spindle or nuclei. The significance of these observations on the role of MFs at the M/G1 interface is discussed.

1 Corresponding author: E-mail, hasezawa{at}k.u-tokyo.ac.jp; Fax, +81-4-7136-3706.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
D. Van Damme, S. Coutuer, R. De Rycke, F.-Y. Bouget, D. Inze, and D. Geelen
Somatic Cytokinesis and Pollen Maturation in Arabidopsis Depend on TPLATE, Which Has Domains Similar to Coat Proteins
PLANT CELL, December 1, 2006; 18(12): 3502 - 3518.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Bannigan, A. M. D. Wiedemeier, R. E. Williamson, R. L. Overall, and T. I. Baskin
Cortical Microtubule Arrays Lose Uniform Alignment Between Cells and are Oryzalin Resistant in the Arabidopsis Mutant, radially swollen 6
Plant Cell Physiol., July 1, 2006; 47(7): 949 - 958.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Oka, Y. Yanagawa, T. Asada, A. Yoneda, S. Hasezawa, T. Sato, and H. Nakagawa
Inhibition of Proteasome by MG-132 Treatment Causes Extra Phragmoplast Formation and Cortical Microtubule Disorganization during M/G1 Transition in Synchronized Tobacco Cells
Plant Cell Physiol., November 15, 2004; 45(11): 1623 - 1632.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.