Plant and Cell Physiology, 2004, Vol. 45, No. 6 684-692
© 2004 Oxford University Press
A Novel Interaction between Calreticulin and Ubiquitin-Like Nuclear Protein in Rice
1 Japan Society for the Promotion of Science, Tsukuba, Ibaraki, 305-8602 Japan
2 National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602 Japan
3 Department of Applied Biological Science, Tokyo University of Science, Chiba, 278-8510 Japan
Calreticulin (CRT), a major Ca2+-sequestering protein, has been implicated in a variety of cellular functions such as Ca2+ storage, signaling and chaperone activity within the cytoplasm and endoplasmic reticulum. To investigate the biological role of CRT in rice, 21 partial cDNAs, encoding proteins that interacted with rice CRT in a yeast two-hybrid interaction-cloning system, were characterized and the nucleotide sequences were found to be identical to each other. A full-length cDNA of 3.5 kb, obtained from rice genomic sequence data and 5' RACE, codes for a novel protein of 966 amino acid residues and was designated as CRTintP (CRT interacting protein). Primary sequence analysis of CRTintP showed no sequence homology with the known functional proteins; however, a potential ubiquitin-like domain at the N-terminal together with a putative leucine zipper, a nuclear localization signal and several sites for serine/threonine kinases were evident. Cellular localization of CRTintP demonstrated its role in directing green fluorescent protein to the nucleus in onion epidermal cells. Northern and immunoblot analysis showed increased expression of CRT and CRTintP in response to cold stress. Co-immunoprecipitation using anti-CRT antibodies confirmed the existence of the CRT-CRTintP complex in vivo in the stressed leaf tissue, suggesting their potential role in regulating stress response.
4 Corresponding author: E-mail, skomatsu{at}affrc.go.jp; Fax, +81-298-38-7464.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Chandran, Y. C. Tai, G. Hather, J. Dewdney, C. Denoux, D. G. Burgess, F. M. Ausubel, T. P. Speed, and M. C. Wildermuth Temporal Global Expression Data Reveal Known and Novel Salicylate-Impacted Processes and Regulators Mediating Powdery Mildew Growth and Reproduction on Arabidopsis Plant Physiology, March 1, 2009; 149(3): 1435 - 1451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Grespin, G. M. C. Bonamy, V. R. Roggero, N. G. Cameron, L. E. Adam, A. P. Atchison, V. M. Fratto, and L. A. Allison Thyroid Hormone Receptor {alpha}1 Follows a Cooperative CRM1/Calreticulin-mediated Nuclear Export Pathway J. Biol. Chem., September 12, 2008; 283(37): 25576 - 25588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-Y. Jia, C.-Y. Xu, R.-L. Jing, R.-Z. Li, X.-G. Mao, J.-P. Wang, and X.-P. Chang Molecular cloning and characterization of wheat calreticulin (CRT) gene involved in drought-stressed responses J. Exp. Bot., March 1, 2008; 59(4): 739 - 751. [Abstract] [Full Text] [PDF]
|
|