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Plant and Cell Physiology, 2004, Vol. 45, No. 6 667-671
© 2004 Oxford University Press

Rapid Paper

Improvement of Culture Conditions and Evidence for Nuclear Transformation by Homologous Recombination in a Red Alga, Cyanidioschyzon merolae 10D

Ayumi Minoda1, Rei Sakagami2, Fumi Yagisawa3,4, Tsuneyoshi Kuroiwa3 and Kan Tanaka1,5

1 Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Tokyo, 113-0032 Japan
2 School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, 192-0392 Japan
3 Department of Life Science, College of Science, Rikkyo (St. Paul’s) University, Nishiikebukuro, Tokyo, 171-8501 Japan
4 Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo, Tokyo, 113-0033 Japan

Although the nuclear genome sequence of Cyanidioschyzon merolae 10D, a unicellular red alga, was recently determined, DNA transformation technology that is important as a model plant system has never been available thus far. In this study, improved culture conditions resulted in a faster growth rate of C. merolae in liquid medium (doubling time = 9.2 h), and colony formation on gellan gum plates. Using these conditions, spontaneous mutants (5-fluoroortic acid resistant) deficient in the UMP synthase gene were isolated. The lesions were then restored by introducing the wild-type UMP synthase gene into the cells suggesting DNA transformation by homologous recombination.

5 Corresponding author: E-mail, kntanaka{at}iam.u-tokyo.ac.jp; Fax, +81-3-5841-8476.


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