Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Pedreira, J.
Right arrow Articles by Zarra, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pedreira, J.
Right arrow Articles by Zarra, I.
Agricola
Right arrow Articles by Pedreira, J.
Right arrow Articles by Zarra, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2004, Vol. 45, No. 5 530-534
© 2004 Oxford University Press

Role of Apoplastic Ascorbate and Hydrogen Peroxide in the Control of Cell Growth in Pine Hypocotyls

Jorge Pedreira, Noelia Sanz, María Jesús Peña1, María Sánchez, Eva Queijeiro, Gloria Revilla and Ignacio Zarra2

Departamento de Fisiología Vegetal, Facultad de Biología, Universidad de Santiago de Compostela, E-15782 Santiago de Compostela, Spain

The growth cessation of plant axis has been related with the formation of diphenyl bridges among the pectic components of the cell wall caused by the action of apoplastic peroxidases using hydrogen peroxide as electron acceptor. The formation of diphenyl bridges is prevented by the presence of ascorbate in the apoplastic fluid which acts as a hydrogen peroxide scavenger. The current work focuses on the role of the apoplastic ascorbate and hydrogen peroxide in the cell growth. The addition of hydrogen peroxide caused an inhibition of the auxin-induced growth as well as a significant decrease in the cell wall creep induced by acid-pH solutions. The hydrogen peroxide content in apoplastic fluid increased with the hypocotyl age and along the hypocotyl axis of 10-day-old pine seedlings, as the growth capacity decreased. On the other hand, the ascorbate content in the apoplastic fluid decreased with the hypocotyl age and along the hypocotyl axis of 10-day-old seedlings. A very significant correlation between the hydrogen peroxide apoplastic level and the growth rate as well as between the ascorbate/hydrogen peroxide molar ratio and the growth rate of hypocotyls have been found suggesting that the redox state is the main factor controlling the cell wall stiffening mechanism and thus growth in pine hypocotyls.

1 Present address: Complex Carbohydrate Research Center, The University of Georgia, Athens, GA, U.S.A.

2 Corresponding author: E-mail, bvzarra{at}usc.es; Fax, +34-981-596904.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
G. B. Monshausen, T. N. Bibikova, M. A. Messerli, C. Shi, and S. Gilroy
From the Cover: Oscillations in extracellular pH and reactive oxygen species modulate tip growth of Arabidopsis root hairs
PNAS, December 26, 2007; 104(52): 20996 - 21001.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. R. Knight
New ideas on root hair growth appear from the flanks
PNAS, December 26, 2007; 104(52): 20649 - 20650.
[Full Text] [PDF]

Home page
 J Exp BotHome page
A. Karkonen and S. C Fry
Effect of ascorbate and its oxidation products on H2O2 production in cell-suspension cultures of Picea abies and in the absence of cells
J. Exp. Bot., May 1, 2006; 57(8): 1633 - 1644.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
S. G. WI, A. P. SINGH, K. H. LEE, and Y. S. KIM
The Pattern of Distribution of Pectin, Peroxidase and Lignin in the Middle Lamella of Secondary Xylem Fibres in Alfalfa (Medicago sativa)
Ann. Bot., April 1, 2005; 95(5): 863 - 868.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.