Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hashimoto, K.
Right arrow Articles by Iida, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hashimoto, K.
Right arrow Articles by Iida, H.
Agricola
Right arrow Articles by Hashimoto, K.
Right arrow Articles by Iida, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2004, Vol. 45, No. 4 496-500
© 2004 Oxford University Press

Short Communication

Functional Analysis of a Rice Putative Voltage-Dependent Ca2+ Channel, OsTPC1, Expressed in Yeast Cells Lacking its Homologous Gene CCH1

Kenji Hashimoto1, Mikako Saito1,4, Hideaki Matsuoka1,4,5, Kazuko Iida2 and Hidetoshi Iida3,4

1 Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo, 184-8588 Japan
2 Medical R & D Center, The Tokyo Metropolitan Institute of Medical Science, Honkomagome 3-18-22, Bunkyo-ku, Tokyo, 113-8613 Japan
3 Department of Biology, Tokyo Gakugei University, Tokyo, 184-8501 Japan
4 CREST, Japan Science and Technology Agency, Saitama, 332-0012 Japan

Abstract

We isolated a cDNA (OsTPC1) from rice that was homologous to AtTPC1, a putative voltage-dependent Ca2+ channel (VDCC) gene of Arabidopsis thaliana. The hydropathy profile of its deduced amino acid sequence showed significant structural features of the {alpha}1-subunit of animal VDCCs. Functional analysis using a heterologous yeast expression system showed that OsTPC1 facilitates Ca2+ permeation. The Km value for Ca2+ of OsTPC1, 47.5 µM, was comparable to that of intrinsic CCH1, a candidate VDCC in yeast. Ca2+ permeation by OsTPC1 was inhibited by verapamil, a VDCC blocker. These findings indicate for the first time that OsTPC1 is a putative VDCC in rice.

Footnotes

5 Corresponding author: E-mail, bio-func{at}cc.tuat.ac.jp; Fax, +81-42–387-1503.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
I. I. Pottosin and G. Schonknecht
Vacuolar calcium channels
J. Exp. Bot., May 1, 2007; 58(7): 1559 - 1569.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y.-J. Wang, J.-N. Yu, T. Chen, Z.-G. Zhang, Y.-J. Hao, J.-S. Zhang, and S.-Y. Chen
Functional analysis of a putative Ca2+ channel gene TaTPC1 from wheat
J. Exp. Bot., December 1, 2005; 56(422): 3051 - 3060.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Z.-l. Shang, L.-g. Ma, H.-l. Zhang, R.-r. He, X.-c. Wang, S.-j. Cui, and D.-y. Sun
Ca2+ Influx into Lily Pollen Grains Through a Hyperpolarization-activated Ca2+-permeable Channel Which Can be Regulated by Extracellular CaM
Plant Cell Physiol., April 1, 2005; 46(4): 598 - 608.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.