Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (25)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Wong, J. H.
Right arrow Articles by Buchanan, B. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wong, J. H.
Right arrow Articles by Buchanan, B. B.
Agricola
Right arrow Articles by Wong, J. H.
Right arrow Articles by Buchanan, B. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2004, Vol. 45, No. 4 407-415
© 2004 Oxford University Press

This paper is dedicated to Professor Keishiro Wada to commemorate his retirement from the University of Kanazawa. Work with Professor Wada on purothionin provided the first evidence for a role for thioredoxin in seeds (Wada and Buchanan 1981, Johnson et al. 1987).

Thioredoxin Reduction Alters the Solubility of Proteins of Wheat Starchy Endosperm: An Early Event in Cereal Germination

Joshua H. Wong1, Nick Cai1, Charlene K. Tanaka2, William H. Vensel2, William J. Hurkman2 and Bob B. Buchanan1,3

1 Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720, U.S.A.
2 USDA, Agricultural Research Service, Western Regional Research Center, Albany, CA 94710, U.S.A.

A KCl-soluble, albumin/globulin fraction of wheat (Triticum aestivum L.) starchy endosperm was further separated into a methanol-insoluble fraction that contained metabolic proteins and a methanol-soluble fraction that contained "chloroform-methanol" or CM-like proteins. Reduction of the disulfide bonds of the CM proteins with thioredoxin or dithiothreitol altered their properties so that, like the metabolic proteins, they were insoluble in methanol. Glutathione had little effect, indicating dithiol specificity. Proteomic analysis of the CM protein fraction revealed the presence of isoforms of low molecular weight disulfide proteins ({alpha}-amylase, {alpha}-amylase/trypsin and WCI proteinase inhibitors, lipid transfer proteins, {gamma}-thionins), stress enzymes (Cu-Zn superoxide dismutase and peroxidase), storage proteins ({alpha}-, {gamma}- and {omega}-gliadins, low molecular weight glutenin subunits and globulins of the avenin N9 type), and a component of protein degradation (polyubiquitin). These findings support the view that, in addition to modifying activity and increasing protease sensitivity, reduction by thioredoxin alters protein solubility, thereby promoting processes of the grain starchy endosperm, notably the mobilization of reserves during germination and seedling development.

3 Corresponding author: E-mail, view{at}nature.berkeley.edu; Fax, +1-510-642-7356.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol PlantHome page
A. Shahpiri, B. Svensson, and C. Finnie
From Proteomics to Structural Studies of Cytosolic/Mitochondrial-Type Thioredoxin Systems in Barley Seeds
Mol Plant, May 1, 2009; 2(3): 378 - 389.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
Y.-C. Li, J.-P. Ren, M.-J. Cho, S.-M. Zhou, Y.-B. Kim, H.-X. Guo, J. H. Wong, H.-B. Niu, H.-K. Kim, S. Morigasaki, et al.
The Level of Expression of Thioredoxin is Linked to Fundamental Properties and Applications of Wheat Seeds
Mol Plant, May 1, 2009; 2(3): 430 - 441.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Shahpiri, B. Svensson, and C. Finnie
The NADPH-Dependent Thioredoxin Reductase/Thioredoxin System in Germinating Barley Seeds: Gene Expression, Protein Profiles, and Interactions between Isoforms of Thioredoxin h and Thioredoxin Reductase
Plant Physiology, February 1, 2008; 146(2): 789 - 799.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Alkhalfioui, M. Renard, W. H. Vensel, J. Wong, C. K. Tanaka, W. J. Hurkman, B. B. Buchanan, and F. Montrichard
Thioredoxin-Linked Proteins Are Reduced during Germination of Medicago truncatula Seeds
Plant Physiology, July 1, 2007; 144(3): 1559 - 1579.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. Cazalis, P. Pulido, T. Aussenac, J. M. Perez-Ruiz, and F. J. Cejudo
Cloning and characterization of three thioredoxin h isoforms from wheat showing differential expression in seeds
J. Exp. Bot., July 1, 2006; 57(10): 2165 - 2172.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Balmer, W. H Vensel, F. M DuPont, B. B Buchanan, and W. J Hurkman
Proteome of amyloplasts isolated from developing wheat endosperm presents evidence of broad metabolic capability
J. Exp. Bot., April 1, 2006; 57(7): 1591 - 1602.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Job, L. Rajjou, Y. Lovigny, M. Belghazi, and D. Job
Patterns of Protein Oxidation in Arabidopsis Seeds and during Germination
Plant Physiology, June 1, 2005; 138(2): 790 - 802.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.