Plant and Cell Physiology, 2004, Vol. 45, No. 4 369-385
© 2004 Oxford University Press
Rapid Paper |
amphivasal vascular bundle 1, a Gain-of-Function Mutation of the IFL1/REV Gene, Is Associated with Alterations in the Polarity of Leaves, Stems and Carpels
Department of Plant Biology, University of Georgia, Athens, GA 30602, U.S.A.
In Arabidopsis stems, the vascular bundles in the stele are arranged in a ring-like pattern and the vascular tissues in each bundle are organized in a collateral pattern. We have shown previously that the semidominant amphivasal vascular bundle 1 (avb1) mutation transforms the collateral vascular bundles into amphivasal bundles and disrupts the ring-like arrangement of vascular bundles in the stele. In this study, we show that the avb1 mutation occurred in the putative microRNA 165 target sequence in the IFL1/REV gene and caused an amino acid substitution in the putative sterol/lipid-binding START domain. We present direct evidence that the wild-type IFL1/REV mRNA was cleaved within the microRNA 165 target sequence and the avb1 mutation resulted in an inhibition of cleavage and a higher level accumulation of full-length mRNA, suggesting a role of microRNA 165 in the regulation of IFL1/REV gene expression. In addition to an alteration in vascular patterning, the avb1 mutation also caused dramatic changes in fiber cell wall thickening and organ polarity, including aberrant formation and proliferation of cauline leaves and branches, production of trumpet-shaped leaves with reversed adaxial-abaxial identity, ectopic growth of carpel-like structures on the outer surface of carpels, and fasciation of inflorescence. Ectopic overexpression of the avb1 mutant cDNA not only phenocopied most of the avb1 mutant phenotypes but also led to additional novel phenotypes such as formation of leaves with extremely narrow blades and ectopic production of branches in the axil of siliques. Taken together, these results suggest that the avb1 gain-of-function mutation of the IFL1/REV gene alters the positional information that determines vascular patterning and organ polarity.
1 Corresponding author: E-mail, zhye{at}plantbio.uga.edu; Fax, +1-706-542-1805.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  W. Ckurshumova, K. Koizumi, S. P. Chatfield, S. U. Sanchez-Buelna, A. E. Gangaeva, R. McKenzie, and T. Berleth Tissue-Specific GAL4 Expression Patterns as a Resource Enabling Targeted Gene Expression, Cell Type-Specific Transcript Profiling and Gene Function Characterization in the Arabidopsis Vascular System Plant Cell Physiol., January 1, 2009; 50(1): 141 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Candela, R. Johnston, A. Gerhold, T. Foster, and S. Hake The milkweed pod1 Gene Encodes a KANADI Protein That Is Required for Abaxial/Adaxial Patterning in Maize Leaves PLANT CELL, August 1, 2008; 20(8): 2073 - 2087. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-I. Itoh, K.-I. Hibara, Y. Sato, and Y. Nagato Developmental Role and Auxin Responsiveness of Class III Homeodomain Leucine Zipper Gene Family Members in Rice Plant Physiology, August 1, 2008; 147(4): 1960 - 1975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Pinon, J. P. Etchells, P. Rossignol, S. A. Collier, J. M. Arroyo, R. A. Martienssen, and M. E. Byrne Three PIGGYBACK genes that specifically influence leaf patterning encode ribosomal proteins Development, April 1, 2008; 135(7): 1315 - 1324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yao, Q. Ling, H. Wang, and H. Huang Ribosomal proteins promote leaf adaxial identity Development, April 1, 2008; 135(7): 1325 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-S. Kim, S.-G. Kim, M. Lee, I. Lee, H.-Y. Park, P. J. Seo, J.-H. Jung, E.-J. Kwon, S. W. Suh, K.-H. Paek, et al. HD-ZIP III Activity Is Modulated by Competitive Inhibitors via a Feedback Loop in Arabidopsis Shoot Apical Meristem Development PLANT CELL, April 1, 2008; 20(4): 920 - 933. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Baucher, M. El Jaziri, and O. Vandeputte From primary to secondary growth: origin and development of the vascular system J. Exp. Bot., October 1, 2007; 58(13): 3485 - 3501. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Nagasaki, J.-i. Itoh, K. Hayashi, K.-i. Hibara, N. Satoh-Nagasawa, M. Nosaka, M. Mukouhata, M. Ashikari, H. Kitano, M. Matsuoka, et al. The small interfering RNA production pathway is required for shoot meristem initiation in rice PNAS, September 11, 2007; 104(37): 14867 - 14871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Fu, L. Xu, B. Xu, L. Yang, Q. Ling, H. Wang, and H. Huang Genetic Interactions Between Leaf Polarity-Controlling Genes and ASYMMETRIC LEAVES1 and 2 in Arabidopsis Leaf Patterning Plant Cell Physiol., May 1, 2007; 48(5): 724 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Chitwood, M. Guo, F. T. S. Nogueira, and M. C. P. Timmermans Establishing leaf polarity: the role of small RNAs and positional signals in the shoot apex Development, March 1, 2007; 134(5): 813 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-K. Zhou, M. Kubo, R. Zhong, T. Demura, and Z.-H. Ye Overexpression of miR165 Affects Apical Meristem Formation, Organ Polarity Establishment and Vascular Development in Arabidopsis Plant Cell Physiol., March 1, 2007; 48(3): 391 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Bove, C. L.H. Hord, and M. A. Mullen The blossoming of RNA biology: Novel insights from plant systems RNA, December 1, 2006; 12(12): 2035 - 2046. [Full Text] [PDF]
|
|
|
|
|
|
S. Nagawa, S. Sawa, S. Sato, T. Kato, S. Tabata, and H. Fukuda Gene Trapping in Arabidopsis Reveals Genes Involved in Vascular Development Plant Cell Physiol., October 1, 2006; 47(10): 1394 - 1405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Huang, L. Pi, W. Liang, B. Xu, H. Wang, R. Cai, and H. Huang The Proteolytic Function of the Arabidopsis 26S Proteasome Is Required for Specifying Leaf Adaxial Identity PLANT CELL, October 1, 2006; 18(10): 2479 - 2492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Xu, L. Yang, L. Pi, Q. Liu, Q. Ling, H. Wang, R. S. Poethig, and H. Huang Genetic Interaction between the AS1-AS2 and RDR6-SGS3-AGO7 Pathways for Leaf Morphogenesis Plant Cell Physiol., July 1, 2006; 47(7): 853 - 863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Ochando, S. Jover-Gil, J. J. Ripoll, H. Candela, A. Vera, M. R. Ponce, A. Martinez-Laborda, and J. L. Micol Mutations in the MicroRNA Complementarity Site of the INCURVATA4 Gene Perturb Meristem Function and Adaxialize Lateral Organs in Arabidopsis Plant Physiology, June 1, 2006; 141(2): 607 - 619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Floyd, C. S. Zalewski, and J. L. Bowman Evolution of Class III Homeodomain-Leucine Zipper Genes in Streptophytes Genetics, May 1, 2006; 173(1): 373 - 388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Inagaki, T. Suzuki, M.-a. Ohto, H. Urawa, T. Horiuchi, K. Nakamura, and A. Morikami Arabidopsis TEBICHI, with Helicase and DNA Polymerase Domains, Is Required for Regulated Cell Division and Differentiation in Meristems PLANT CELL, April 1, 2006; 18(4): 879 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ohashi-Ito, M. Kubo, T. Demura, and H. Fukuda Class III Homeodomain Leucine-Zipper Proteins Regulate Xylem Cell Differentiation Plant Cell Physiol., October 1, 2005; 46(10): 1646 - 1656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Williams, S. P. Grigg, M. Xie, S. Christensen, and J. C. Fletcher Regulation of Arabidopsis shoot apical meristem and lateral organ formation by microRNA miR166g and its AtHD-ZIP target genes Development, August 15, 2005; 132(16): 3657 - 3668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Li, L. Xu, H. Wang, Z. Yuan, X. Cao, Z. Yang, D. Zhang, Y. Xu, and H. Huang The Putative RNA-Dependent RNA Polymerase RDR6 Acts Synergistically with ASYMMETRIC LEAVES1 and 2 to Repress BREVIPEDICELLUS and MicroRNA165/166 in Arabidopsis Leaf Development PLANT CELL, August 1, 2005; 17(8): 2157 - 2171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Xie, E. Allen, N. Fahlgren, A. Calamar, S. A. Givan, and J. C. Carrington Expression of Arabidopsis MIRNA Genes Plant Physiology, August 1, 2005; 138(4): 2145 - 2154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Axtell and D. P. Bartel Antiquity of MicroRNAs and Their Targets in Land Plants PLANT CELL, June 1, 2005; 17(6): 1658 - 1673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zhao, J. C. Craig, H. E. Petzold, A. W. Dickerman, and E. P. Beers The Xylem and Phloem Transcriptomes from Secondary Tissues of the Arabidopsis Root-Hypocotyl Plant Physiology, June 1, 2005; 138(2): 803 - 818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Mallory, D. P. Bartel, and B. Bartel MicroRNA-Directed Regulation of Arabidopsis AUXIN RESPONSE FACTOR17 Is Essential for Proper Development and Modulates Expression of Early Auxin Response Genes PLANT CELL, May 1, 2005; 17(5): 1360 - 1375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Prigge, D. Otsuga, J. M. Alonso, J. R. Ecker, G. N. Drews, and S. E. Clark Class III Homeodomain-Leucine Zipper Gene Family Members Have Overlapping, Antagonistic, and Distinct Roles in Arabidopsis Development PLANT CELL, January 1, 2005; 17(1): 61 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.C.P. TIMMERMANS, M.T. JUAREZ, and T.L. PHELPS-DURR A Conserved microRNA Signal Specifies Leaf Polarity Cold Spring Harb Symp Quant Biol, January 1, 2004; 69(0): 409 - 418. [Abstract] [PDF]
|
|