Plant and Cell Physiology, 2004, Vol. 45, No. 3 340-345
© 2004 Oxford University Press
Short Communication |
Induction of SULTR1;1 Sulfate Transporter in Arabidopsis Roots Involves Protein Phosphorylation/Dephosphorylation Circuit for Transcriptional Regulation
1 RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045 Japan
2 Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555 Japan
Abstract
SULTR1;1 high-affinity sulfate transporter is highly regulated by sulfur deficiency (–S) in the epidermis and cortex of Arabidopsis roots. The regulatory mechanism of SULTR1;1 expression was studied using inhibitors for transcription, translation, protein phosphorylation and dephosphorylation. The induction of SULTR1;1 mRNA during –S was blocked by the addition of actinomycin D in the medium, suggesting that SULTR1;1 is transcriptionally regulated. Cycloheximide repressed the –S induction of SULTR1;1, but enhanced the basal mRNA level of SULTR1;1 under sulfur replete (+S) condition. In addition, the induction of SULTR1;1 by –S was significantly blocked by okadaic acid (OKA) and calyculin A (CalyA). Regulation of SULTR1;1 was further confirmed in transgenic plants expressing green fluorescent protein (GFP) under the control of SULTR1;1 promoter. Accumulation of GFP during –S was dependent to SULTR1;1 promoter, and the effects of OKA and CalyA were reproducible in the SULTR1;1 promoter-GFP plants. These results suggested that the up-regulation of SULTR1;1 by –S requires protein phosphatase as an upstream regulatory factor.
Footnotes
3 Corresponding author: E-mail, hideki{at}postman.riken.go.jp; Fax, +81-45-503-9609.
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