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Plant and Cell Physiology, 2004, Vol. 45, No. 3 300-308
© 2004 Oxford University Press

Proteomic Analysis on Symbiotic Differentiation of Mitochondria in Soybean Nodules

Le Thi-Phuong Hoa1,2, Mika Nomura1, Hideyuki Kajiwara3, David Alexander Day4 and Shigeyuki Tajima1,5

1 Department of Life Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa, 761-0795 Japan
2 Faculty of Biology-Agriculture, Hanoi University of Education, Km8, XuanThuy Road, Hanoi, Vietnam
3 Department of Biochemistry, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602 Japan
4 School of Biomedical & Chemical Sciences, Faculty of Life & Physical Sciences, University of Western Australia, Stirling Highway, Nedlands 6907, Western Australia, Australia

Symbiotic interactions between legume plants and rhizobia induce specific metabolisms and intracellular organelles in nodules. For surveying symbiotic differentiation of a key organelle, mitochondria, protein constituents of soybean nodule and root mitochondria were compared after two-dimensional (2-D) electrophoresis, and the proteins were characterized in combination with matrix-assisted desorption/ionization time-of-flight mass spectrometry, electrospray ionization mass spectrometry and N-terminal amino acid sequencing. Of the proteins that were detected only in nodule mitochondria, phosphoserine aminotransferase, flavanone 3-hydroxylase, coproporphyrinogen III oxidase, one ribonucleoprotein and three unknown proteins were identified. Seven up-regulated, eight down-regulated and two strongly suppressed protein spots in nodule mitochondria were also assigned protein identities. The physiological roles of these differential expressions were discussed in relation to nodule-specific metabolisms in soybean nodules.

5 Corresponding author: E-mail, tajima{at}ag.kagawa-u.ac.jp; Fax, +81-87-891-3021.


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