Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Zhang, X.
Right arrow Articles by Oppenheimer, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, X.
Right arrow Articles by Oppenheimer, D. G.
Agricola
Right arrow Articles by Zhang, X.
Right arrow Articles by Oppenheimer, D. G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2004, Vol. 45, No. 2 221-224
© 2004 Oxford University Press

Short Communication

A Simple and Efficient Method for Isolating Trichomes for Downstream Analyses

Xiaoguo Zhang and David G. Oppenheimer1

Department of Botany and the University of Florida Genetics Institute, University of Florida, P.O. Box 118526, Gainesville, FL 32611-8526, U.S.A.

Abstract

Arabidopsis trichomes are an excellent cell type to address many questions in plant biology including the control of cell shape, endoreplication, and cell expansion. Because trichomes comprise such a small percentage of the cells of a leaf, biochemical analyses of trichomes are limited. To overcome this limitation, we developed a method for removing trichomes from the leaf surface. Our method allows the isolation of intact trichomes for use in downstream applications such as cell wall analysis, immunolocalization of trichome proteins, analysis of DNA content, and proteomics. Also, this method will facilitate the isolation of trichomes from practically any plant species.

Footnotes

1 Corresponding author: E-mail, doppen{at}botany.ufl.edu; Fax, +1-352-392-3993.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. Dobney, D. Chiasson, P. Lam, S. P. Smith, and W. A. Snedden
The Calmodulin-related Calcium Sensor CML42 Plays a Role in Trichome Branching
J. Biol. Chem., November 13, 2009; 284(46): 31647 - 31657.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
A. Ben Hassine, M. E. Ghanem, S. Bouzid, and S. Lutts
Abscisic acid has contrasting effects on salt excretion and polyamine concentrations of an inland and a coastal population of the Mediterranean xero-halophyte species Atriplex halimus
Ann. Bot., October 1, 2009; 104(5): 925 - 936.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. M. Plett, J. Mathur, and S. Regan
Ethylene receptor ETR2 controls trichome branching by regulating microtubule assembly in Arabidopsis thaliana
J. Exp. Bot., September 1, 2009; 60(13): 3923 - 3933.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Breuer, A. Kawamura, T. Ichikawa, R. Tominaga-Wada, T. Wada, Y. Kondou, S. Muto, M. Matsui, and K. Sugimoto
The Trihelix Transcription Factor GTL1 Regulates Ploidy-Dependent Cell Growth in the Arabidopsis Trichome
PLANT CELL, August 1, 2009; 21(8): 2307 - 2322.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. J. Jakoby, D. Falkenhan, M. T. Mader, G. Brininstool, E. Wischnitzki, N. Platz, A. Hudson, M. Hulskamp, J. Larkin, and A. Schnittger
Transcriptional Profiling of Mature Arabidopsis Trichomes Reveals That NOECK Encodes the MIXTA-Like Transcriptional Regulator MYB106
Plant Physiology, November 1, 2008; 148(3): 1583 - 1602.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Borghi, M. Bureau, and R. Simon
Arabidopsis JAGGED LATERAL ORGANS Is Expressed in Boundaries and Coordinates KNOX and PIN Activity
PLANT CELL, June 1, 2007; 19(6): 1795 - 1808.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Verelst, H. Saedler, and T. Munster
MIKC* MADS-Protein Complexes Bind Motifs Enriched in the Proximal Region of Late Pollen-Specific Arabidopsis Promoters
Plant Physiology, January 1, 2007; 143(1): 447 - 460.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Calo, I. Garcia, C. Gotor, and L. C. Romero
Leaf hairs influence phytopathogenic fungus infection and confer an increased resistance when expressing a Trichoderma {alpha}-1,3-glucanase
J. Exp. Bot., November 1, 2006; 57(14): 3911 - 3920.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. K. Imai, Y. Ohashi, T. Tsuge, T. Yoshizumi, M. Matsui, A. Oka, and T. Aoyama
The A-Type Cyclin CYCA2;3 Is a Key Regulator of Ploidy Levels in Arabidopsis Endoreduplication
PLANT CELL, February 1, 2006; 18(2): 382 - 396.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
X. Zhang, P. H. Grey, S. Krishnakumar, and D. G. Oppenheimer
The IRREGULAR TRICHOME BRANCH loci regulate trichome elongation in Arabidopsis
Plant Cell Physiol., September 1, 2005; 46(9): 1549 - 1560.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
X. Zhang, J. Dyachok, S. Krishnakumar, L. G. Smith, and D. G. Oppenheimer
IRREGULAR TRICHOME BRANCH1 in Arabidopsis Encodes a Plant Homolog of the Actin-Related Protein2/3 Complex Activator Scar/WAVE That Regulates Actin and Microtubule Organization
PLANT CELL, August 1, 2005; 17(8): 2314 - 2326.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.