Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Iwai, M.
Right arrow Articles by Ikeuchi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iwai, M.
Right arrow Articles by Ikeuchi, M.
Agricola
Right arrow Articles by Iwai, M.
Right arrow Articles by Ikeuchi, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2004, Vol. 45, No. 2 171-175
© 2004 Oxford University Press

Improved Genetic Transformation of the Thermophilic Cyanobacterium, Thermosynechococcus elongatus BP-1

Masako Iwai, Hiroshi Katoh, Mitsunori Katayama and Masahiko Ikeuchi1

Department of Life Sciences (Biology), The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo, 153-8902 Japan

We improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1, by combining electroporation with a top agar method. Transformation was also improved when a disruptant of a putative type I restriction endonuclease (tll2230) was used as recipient cells. In particular, some constructs, with which wild type has never been transformed, were successfully integrated into the tll2230-disruptant. Single-crossover recombination was detected more frequently than the double-crossover recombination. In accordance with the presence of all the homologs of pil genes in Synechocystis sp. PCC 6803, we found that T. elongatus is naturally transformable with exogenous DNA.

1 Corresponding author: E-mail, mikeuchi{at}bio.c.u-tokyo.ac.jp; Fax, +81-3-5454-4337.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
M. Iwai, T. Maoka, M. Ikeuchi, and S. Takaichi
2,2'-{beta}-Hydroxylase (CrtG) is Involved in Carotenogenesis of Both Nostoxanthin and 2-Hydroxymyxol 2'-Fucoside in Thermosynechococcus elongatus Strain BP-1
Plant Cell Physiol., November 1, 2008; 49(11): 1678 - 1687.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
D. G. Adams and P. S. Duggan
Cyanobacteria-bryophyte symbioses
J. Exp. Bot., March 1, 2008; 59(5): 1047 - 1058.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Iwai, T. Suzuki, N. Dohmae, Y. Inoue, and M. Ikeuchi
Absence of the PsbZ Subunit Prevents Association of PsbK and Ycf12 with the PSII Complex in the Thermophilic Cyanobacterium Thermosynechococcus elongatus BP-1
Plant Cell Physiol., December 1, 2007; 48(12): 1758 - 1763.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
O. Zhaxybayeva, J. P. Gogarten, R. L. Charlebois, W. F. Doolittle, and R. T. Papke
Phylogenetic analyses of cyanobacterial genomes: Quantification of horizontal gene transfer events
Genome Res., September 1, 2006; 16(9): 1099 - 1108.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
K. Nakasugi and B. A. Neilan
Identification of Pilus-Like Structures and Genes in Microcystis aeruginosa PCC7806
Appl. Envir. Microbiol., November 1, 2005; 71(11): 7621 - 7625.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Iwai, H. Katoh, M. Katayama, and M. Ikeuchi
PSII-Tc Protein Plays an Important Role in Dimerization of Photosystem II
Plant Cell Physiol., December 15, 2004; 45(12): 1809 - 1816.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.