Plant and Cell Physiology, 2004, Vol. 45, No. 2 160-170
© 2004 Oxford University Press
Cryptogein-Induced Initial Events in Tobacco BY-2 Cells: Pharmacological Characterization of Molecular Relationship among Cytosolic Ca2+ Transients, Anion Efflux and Production of Reactive Oxygen Species
1 Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510 Japan
2 Genome & Drug Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510 Japan
3 Graduate School of Bioagricultural Sciences and Nagoya University Bioscience Center, Nagoya University, Chikusa-ku, Nagoya, 464-8610 Japan
Ion fluxes and the production of reactive oxygen species (ROS) are early events that follow elicitor treatment or microbial infection. However, molecular mechanisms for these responses as well as their relationship have been controversial and still largely unknown. We here simultaneously monitored the temporal sequence of initial events at the plasma membrane in suspension-cultured tobacco cells (cell line BY-2) in response to a purified proteinaceous elicitor, cryptogein, which induced hypersensitive cell death. The elicitor induced transient rise in cytosolic Ca2+ concentration ([Ca2+]cyt) showing two distinct peaks, followed by biphasic (rapid/transient and slow/prolonged) Cl– efflux and H+ influx. Pharmacological analyses suggested that the two phases of the [Ca2+]cyt response correspond to Ca2+ influx through the plasma membrane and an inositol 1,4,5-trisphophate-mediated release of Ca2+ from intracellular Ca2+ stores, respectively, and the [Ca2+]cyt transients and the Cl– efflux were mutually dependent events regulated by protein phosphorylation. The elicitor also induced production of ROS including •O2– and H2O2, which initiated after the [Ca2+]cyt rise and required Ca2+ influx, Cl– efflux and protein phosphorylation. An inhibitor of NADPH oxidase, diphenylene iodonium, completely inhibited the elicitor-induced production of •O2– and H2O2, but did not affect the [Ca2+]cyt transients. These results suggest that cryptogein-induced plasma membrane Ca2+ influx is independent of ROS, and NADPH oxidase dependent ROS production is regulated by these series of ion fluxes.
* The authors would like to dedicate this article to the memory of Dr. Muto, who died on 23 January 2004.
4 Corresponding author: E-mail, kuchitsu{at}rs.noda.tus.ac.jp; Fax, +81-4-7123-9767.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Ogasawara, H. Kaya, G. Hiraoka, F. Yumoto, S. Kimura, Y. Kadota, H. Hishinuma, E. Senzaki, S. Yamagoe, K. Nagata, et al. Synergistic Activation of the Arabidopsis NADPH Oxidase AtrbohD by Ca2+ and Phosphorylation J. Biol. Chem., April 4, 2008; 283(14): 8885 - 8892. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Higaki, T. Goh, T. Hayashi, N. Kutsuna, Y. Kadota, S. Hasezawa, T. Sano, and K. Kuchitsu Elicitor-Induced Cytoskeletal Rearrangement Relates to Vacuolar Dynamics and Execution of Cell Death: In Vivo Imaging of Hypersensitive Cell Death in Tobacco BY-2 Cells Plant Cell Physiol., October 1, 2007; 48(10): 1414 - 1425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ashtamker, V. Kiss, M. Sagi, O. Davydov, and R. Fluhr Diverse Subcellular Locations of Cryptogein-Induced Reactive Oxygen Species Production in Tobacco Bright Yellow-2 Cells Plant Physiology, April 1, 2007; 143(4): 1817 - 1826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Takabatake, E. Karita, S. Seo, I. Mitsuhara, K. Kuchitsu, and Y. Ohashi Pathogen-Induced Calmodulin Isoforms in Basal Resistance Against Bacterial and Fungal Pathogens in Tobacco Plant Cell Physiol., March 1, 2007; 48(3): 414 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kadota, S. Fujii, Y. Ogasawara, Y. Maeda, K. Higashi, and K. Kuchitsu Continuous Recognition of the Elicitor Signal for Several Hours is Prerequisite for Induction of Cell Death and Prolonged Activation of Signaling Events in Tobacco BY-2 Cells Plant Cell Physiol., September 1, 2006; 47(9): 1337 - 1342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yoda, Y. Hiroi, and H. Sano Polyamine Oxidase Is One of the Key Elements for Oxidative Burst to Induce Programmed Cell Death in Tobacco Cultured Cells Plant Physiology, September 1, 2006; 142(1): 193 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sagi and R. Fluhr Production of Reactive Oxygen Species by Plant NADPH Oxidases Plant Physiology, June 1, 2006; 141(2): 336 - 340. [Full Text] [PDF]
|
|
|
|
|
|
N. Beffagna, B. Buffoli, and C. Busi Modulation of Reactive Oxygen Species Production During Osmotic Stress in Arabidopsis thaliana Cultured Cells: Involvement of the Plasma Membrane Ca2+-ATPase and H+-ATPase Plant Cell Physiol., August 1, 2005; 46(8): 1326 - 1339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Tegg, L. Melian, C. R. Wilson, and S. Shabala Plant Cell Growth and Ion Flux Responses to the Streptomycete Phytotoxin Thaxtomin A: Calcium and Hydrogen Flux Patterns Revealed by the Non-invasive MIFE Technique Plant Cell Physiol., April 1, 2005; 46(4): 638 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kadota, T. Watanabe, S. Fujii, Y. Maeda, R. Ohno, K. Higashi, T. Sano, S. Muto, S. Hasezawa, and K. Kuchitsu Cell Cycle Dependence of Elicitor-induced Signal Transduction in Tobacco BY-2 Cells Plant Cell Physiol., January 15, 2005; 46(1): 156 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Karita, H. Yamakawa, I. Mitsuhara, K. Kuchitsu, and Y. Ohashi Three Types of Tobacco Calmodulins Characteristically Activate Plant NAD Kinase at Different Ca2+ Concentrations and pHs Plant Cell Physiol., October 15, 2004; 45(10): 1371 - 1379. [Abstract] [Full Text] [PDF]
|
|