© 2004 Oxford University Press
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A Comprehensive Expression Analysis of the Arabidopsis Proline-rich Extensin-like Receptor Kinase Gene Family using Bioinformatic and Experimental Approaches
1 Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2
2 Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637, USA
The Arabidopsis proline-rich extensin-like receptor kinase (PERK) family consists of 15 predicted receptor kinases. A comprehensive expression analysis was undertaken to identify overlapping and unique expression patterns within this family relative to their phylogeny. Three different approaches were used to study AtPERK gene family expression, and included analyses of the EST, MPSS and NASCArrays databases as well as experimental RNA blot analyses. Some of the AtPERK members were identified as tissue-specific genes while others were more broadly expressed. While in some cases there was a good association between these different expression patterns and the position of the AtPERK members in the kinase phylogeny, in other cases divergence of expression patterns was seen. The PERK expression data identified by the bioinformatics and experimental approaches were found generally to show similar trends and supported the use of data from large-scale expression studies for obtaining preliminary expression data. Thus, the bioinformatics survey for ESTs and microarrays is a powerful comprehensive approach for obtaining a genome-wide view of genes in a multigene family.
3 These authors contributed equally to this work.
4 Present address: Sunnybrook and Women’s College Health Sciences Center, Toronto, Ontario, Canada M4N 3M5.
5 Present address: Plant Biotechnology Institute, Saskatoon, Saskatchewan, Canada S7N 0W9.
6 Present address: Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1.
7 Corresponding author: E-mail, goring{at}botany.utoronto.ca; Fax: +1-416-978-5878.
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