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Plant and Cell Physiology 2004 45(12):1779-1786; doi:10.1093/pcp/pch200
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© 2004 Oxford University Press

Sorting Signals in the Cytosolic Tail of Plant p24 Proteins Involved in the Interaction with the COPII Coat

Inmaculada Contreras1,3, Yaodong Yang2,3, David G. Robinson2 and Fernando Aniento1,4

1 Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad de Valencia. Avda Vicente Andrés Estellés, s/n, E-46100 Burjassot, Valencia. Spain
2 Heidelberg Institute for Plant Sciences, University of Heidelberg. Germany

The ability of the cytosolic tail of a plant p24 protein to bind COPI and COPII subunits from plant and animal sources in vitro has been examined. We have found that a dihydrophobic motif in the –7,–8 position (relative to the cytosolic carboxy-terminus), which strongly cooperates with a dilysine motif in the –3,–4 position for COPI binding, is required for COPII binding. In addition, we show that COPI and COPII coat proteins from plant cytosol compete for binding to the sorting motifs in these tails. Only in the absence of the dilysine motif in the –3,–4 position or after COPI depletion could we observe COPII binding to the p24 tail. This competition is not observed when using rat liver cytosol.

3 Both authors contributed equally to the work.

4 Corresponding author: E-mail, Fernando.aniento{at}uv.es; Fax, +34-963544917.


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