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Plant and Cell Physiology Advance Access originally published online on November 22, 2004
Plant and Cell Physiology 2004 45(12):1759-1767; doi:10.1093/pcp/pch193
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© 2004 Oxford University Press

Azetidine-induced Accumulation of Class I Small Heat Shock Proteins in the Soluble Fraction Provides Thermotolerance in Soybean Seedlings

Tsung-Luo Jinn1, Chi-Chou Chiu1,2, Wei-Wen Song, Yih-Ming Chen and Chu-Yung Lin3

Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan, R.O.C.

Accumulation of class I small heat shock proteins (sHSPs) is induced by the proline analog, azetidine-2-carboxylic acid (Aze) in soybean seedlings to a level similar to that induced by exposure to 40°C. However, only the treatment with 10 mM Aze for 6 h and subsequently with 10 mM proline for 24 h protected the seedlings from damage during subsequent exposure to 45°C as assessed by 2,3,5-triphenyltetrazolium chloride (TTC) staining. A chaperone activity assay showed that the purified class I sHSPs induced by Aze were functional in vitro and protected proteins from thermal denaturation. Amino acid composition analysis indicated that Aze was not incorporated into de novo synthesized class I sHSPs. Accumulation of class I sHSPs in the soluble post-ribosomal supernatant fraction was found to be important for acquisition of thermotolerance. We suggest that both the accumulation of class I sHSPs and their presence in the soluble fraction are important for establishment of thermotolerance.

1 These two authors contributed equally to this work.

2 Present address: Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 112, Taiwan, R.O.C.

3 Corresponding author: E-mail, chuyung{at}ccms.ntu.edu.tw; Fax, +886-2-23638598.


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