© 2004 Oxford University Press
Rapid Paper |
Zinc Transporter of Arabidopsis thaliana AtMTP1 is Localized to Vacuolar Membranes and Implicated in Zinc Homeostasis
1 Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan
2 Graduate School of Human and Environmental Studies, Kyoto University, Kyoto, 606-8501 Japan
3 Department of Biology, Faculty of Science, Kobe University, Kobe, 657-8501 Japan
4 Department of Molecular and Functional Genomics, Shimane University, Matsue, 690-8504 Japan
Cation diffusion facilitator (CDF) proteins belong to a family of heavy metal efflux transporters that might play an essential role in homeostasis and tolerance to metal ions. We investigated the subcellular localization of Arabidopsis thaliana AtMTP1, a member of the CDF family, and its physiological role in the tolerance to Zn using MTP1-deficient mutant plants. AtMTP1 was immunochemically detected as a 43 kDa protein in the vacuolar membrane fractioned by sucrose density gradient centrifugation. The expression level of AtMTP1 in suspension-cultured cells was not affected by the Zn concentration in the medium. When AtMTP1 fused with green fluorescent protein was transiently expressed in protoplasts prepared from Arabidopsis suspension-cultured cells, green fluorescence was clearly observed in the vacuolar membrane. A T-DNA insertion mutant line for AtMTP1 displays enhanced sensitivity to high Zn concentrations ranging from 200 to 500 µM, but not to Zn-deficient conditions. Mesophyll cells of the mtp1-1 mutant plants grown in the presence of 500 µM Zn were degraded, suggesting that Zn at high concentrations causes serious damage to leaves and that AtMTP1 plays a crucial role in preventing this damage in plants. Thus we propose that AtMTP1 is localized in the vacuolar membrane and is involved in sequestration of excess Zn in the cytoplasm into vacuoles to maintain Zn homeostasis.
5 Corresponding author: E-mail: maeshima{at}agr.nagoya-u.ac.jp; Fax, +81-52-789-4096.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Kawachi, Y. Kobae, H. Mori, R. Tomioka, Y. Lee, and M. Maeshima A Mutant Strain Arabidopsis thaliana that Lacks Vacuolar Membrane Zinc Transporter MTP1 Revealed the Latent Tolerance to Excessive Zinc Plant Cell Physiol., June 1, 2009; 50(6): 1156 - 1170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Tennstedt, D. Peisker, C. Bottcher, A. Trampczynska, and S. Clemens Phytochelatin Synthesis Is Essential for the Detoxification of Excess Zinc and Contributes Significantly to the Accumulation of Zinc Plant Physiology, February 1, 2009; 149(2): 938 - 948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shoji, K. Inai, Y. Yazaki, Y. Sato, H. Takase, N. Shitan, K. Yazaki, Y. Goto, K. Toyooka, K. Matsuoka, et al. Multidrug and Toxic Compound Extrusion-Type Transporters Implicated in Vacuolar Sequestration of Nicotine in Tobacco Roots Plant Physiology, February 1, 2009; 149(2): 708 - 718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Iwata, N. V. Fedoroff, and N. Koizumi Arabidopsis bZIP60 Is a Proteolysis-Activated Transcription Factor Involved in the Endoplasmic Reticulum Stress Response PLANT CELL, November 1, 2008; 20(11): 3107 - 3121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamamoto, D. Maruyama, T. Endo, and S.-i. Nishikawa Arabidopsis thaliana Has a Set of J Proteins in the Endoplasmic Reticulum that are Conserved from Yeast to Animals and Plants Plant Cell Physiol., October 1, 2008; 49(10): 1547 - 1562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Klein, H. Sekimoto, M. J. Milner, and L. V. Kochian Investigation of Heavy Metal Hyperaccumulation at the Cellular Level: Development and Characterization of Thlaspi caerulescens Suspension Cell Lines Plant Physiology, August 1, 2008; 147(4): 2006 - 2016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kawachi, Y. Kobae, T. Mimura, and M. Maeshima Deletion of a Histidine-rich Loop of AtMTP1, a Vacuolar Zn2+/H+ Antiporter of Arabidopsis thaliana, Stimulates the Transport Activity J. Biol. Chem., March 28, 2008; 283(13): 8374 - 8383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Haydon and C. S. Cobbett A Novel Major Facilitator Superfamily Protein at the Tonoplast Influences Zinc Tolerance and Accumulation in Arabidopsis Plant Physiology, April 1, 2007; 143(4): 1705 - 1719. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Jaquinod, F. Villiers, S. Kieffer-Jaquinod, V. Hugouvieux, C. Bruley, J. Garin, and J. Bourguignon A Proteomics Dissection of Arabidopsis thaliana Vacuoles Isolated from Cell Culture Mol. Cell. Proteomics, March 1, 2007; 6(3): 394 - 412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ide, N. Nagasaki, R. Tomioka, M. Suito, T. Kamiya, and M. Maeshima Molecular properties of a novel, hydrophilic cation-binding protein associated with the plasma membrane J. Exp. Bot., March 1, 2007; 58(5): 1173 - 1183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Martinoia, M. Maeshima, and H. E. Neuhaus Vacuolar transporters and their essential role in plant metabolism J. Exp. Bot., January 1, 2007; 58(1): 83 - 102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ide, R. Tomioka, Y. Ouchi, T. Kamiya, and M. Maeshima Transcriptional Induction of Two Genes for CCaPs, Novel Cytosolic Proteins, in Arabidopsis thaliana in the Dark Plant Cell Physiol., January 1, 2007; 48(1): 54 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. van de Mortel, L. Almar Villanueva, H. Schat, J. Kwekkeboom, S. Coughlan, P. D. Moerland, E. Ver Loren van Themaat, M. Koornneef, and M. G.M. Aarts Large Expression Differences in Genes for Iron and Zinc Homeostasis, Stress Response, and Lignin Biosynthesis Distinguish Roots of Arabidopsis thaliana and the Related Metal Hyperaccumulator Thlaspi caerulescens Plant Physiology, November 1, 2006; 142(3): 1127 - 1147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mizutani, S. Watanabe, T. Nakagawa, and M. Maeshima Aquaporin NIP2;1 is Mainly Localized to the ER Membrane and Shows Root-Specific Accumulation in Arabidopsis thaliana Plant Cell Physiol., October 1, 2006; 47(10): 1420 - 1426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kobayashi, K. Kawakita, M. Maeshima, N. Doke, and H. Yoshioka Subcellular localization of Strboh proteins and NADPH-dependent O2--generating activity in potato tuber tissues J. Exp. Bot., March 1, 2006; 57(6): 1373 - 1379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kobae, T. Sekino, H. Yoshioka, T. Nakagawa, E. Martinoia, and M. Maeshima Loss of AtPDR8, a Plasma Membrane ABC Transporter of Arabidopsis thaliana, Causes Hypersensitive Cell Death Upon Pathogen Infection Plant Cell Physiol., March 1, 2006; 47(3): 309 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yoshida, M. Kawachi, M. Mori, M. Maeshima, M. Kondo, M. Nishimura, and T. Kondo The Involvement of Tonoplast Proton Pumps and Na+(K+)/H+ Exchangers in the Change of Petal Color During Flower Opening of Morning Glory, Ipomoea tricolor cv. Heavenly Blue Plant Cell Physiol., March 1, 2005; 46(3): 407 - 415. [Abstract] [Full Text] [PDF]
|
|