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Plant and Cell Physiology 2004 45(12):1738-1748; doi:10.1093/pcp/pci009
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© 2004 Oxford University Press

Rapid Paper

Characterization of Full-length Enriched Expressed Sequence Tags of Stress-treated Poplar Leaves

Tokihiko Nanjo1,3, Norihiro Futamura1, Mitsuru Nishiguchi1, Tomohiro Igasaki1, Kazuo Shinozaki2 and Kenji Shinohara1

1 Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, 305-8687 Japan
2 Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, Tsukuba, Ibaraki, 305-0074 Japan

Poplar, whose genome is the first to be sequenced among woody plants, is a favorable model for plant biologists to enable them to understand molecular processes of growth, development and responses to environmental stimuli in trees. The sequence will allow the development of a strategy for improving environmental stress tolerance in forest trees. In this study, we have generated a full-length enriched cDNA library from leaves of axenically grown poplar (Populus nigra var. italica) subjected to environmental stress treatments by dehydration, high salinity, chilling, heat, abscisic acid (ABA) and H2O2. We sequenced >30,000 expressed sequence tags (ESTs) from the cDNA library and consequently collected approximately 4,500 non-redundant clones. We further analyzed cDNAs encoding an ERF/AP2-domain transcription factor which is specific in plants and plays an important role under stress. Thirteen candidates containing the ERF/AP2 domain were found within our EST resource. Some of them showed stress-responsive gene expression. We report here the first collection of full-length enriched stress-related ESTs of poplar and discuss environmental stress responses of forest trees in the light of comparative genomics.

3 Corresponding author: E-mail, nanjo{at}affrc.go.jp; Fax, +81-29-873-0507


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