© 2004 Oxford University Press
Short Communication |
Molecular and Biochemical Characterization of Three WD-Repeat-Domain-containing Inositol Polyphosphate 5-Phosphatases in Arabidopsis thaliana
Department of Plant Biology, University of Georgia, Athens, GA 30602, U.S.A.
Abstract
Type II inositol polyphosphate 5-phosphatases (5PTases) in animals and yeast have been known to be important for regulating inositol and phospholipid signaling by hydrolyzing phosphate from both inositol polyphosphates and phosphoinositides. However, the molecular and biochemical properties of type II 5PTases in plants have not yet been studied. In this report, we show that three Arabidopsis genes, At5PTase12, At5PTase13 and At5PTase14, encode proteins with a 5PTase domain and a WD-repeat domain, a novel combination present only in plant 5PTases. We demonstrate that these genes are differentially expressed in Arabidopsis organs and At5PTase13 is induced in response to ABA and wounding treatments. Our biochemical studies reveal that although both At5PTase12 and At5PTase13 exhibit phosphatase activity toward only Ins(1,4,5)P3, At5PTase14 hydrolyzes phosphate from PI(4,5)P2, PI(3,4,5)P3 and Ins(1,4,5)P3 with the highest substrate affinity toward PI(4,5)P2. All three At5PTases require Mg2+ for their phosphatase activities. Our molecular and biochemical characterization of three WD-repeat-domain-containing At5PTases provides a foundation for further elucidation of their cellular functions in Arabidopsis.
Footnotes
1 Corresponding author: E-mail, zhye{at}plantbio.uga.edu; Fax, +1-706-542-1805.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Gunesekera, J. Torabinejad, J. Robinson, and G. E. Gillaspy Inositol Polyphosphate 5-Phosphatases 1 and 2 Are Required for Regulating Seedling Growth Plant Physiology, March 1, 2007; 143(3): 1408 - 1417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-H. Lin, Y. Wang, B. Mueller-Roeber, C. A. Brearley, Z.-H. Xu, and H.-W. Xue At5PTase13 Modulates Cotyledon Vein Development through Regulating Auxin Homeostasis Plant Physiology, December 1, 2005; 139(4): 1677 - 1691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Williams, J. Torabinejad, E. Cohick, K. Parker, E. J. Drake, J. E. Thompson, M. Hortter, and D. B. DeWald Mutations in the Arabidopsis Phosphoinositide Phosphatase Gene SAC9 Lead to Overaccumulation of PtdIns(4,5)P2 and Constitutive Expression of the Stress-Response Pathway Plant Physiology, June 1, 2005; 138(2): 686 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Zhong, D. H. Burk, C. J. Nairn, A. Wood-Jones, W. H. Morrison III, and Z.-H. Ye Mutation of SAC1, an Arabidopsis SAC Domain Phosphoinositide Phosphatase, Causes Alterations in Cell Morphogenesis, Cell Wall Synthesis, and Actin Organization PLANT CELL, May 1, 2005; 17(5): 1449 - 1466. [Abstract] [Full Text] [PDF]
|
|