© 2004 Oxford University Press
A Mechanosensitive Anion Channel in Arabidopsis thaliana Mesophyll Cells
1 Lab of Visual Information Processing, Centre for Brain and Cognitive Sciences, Institute of Biophysics, CAS, 15 Da Tun Road, Chaoyang District, Beijing 100101, China
2 ICORP Cell Mechanosensing, Japan Science and Technology Agency (JST), Saitama, 332-0012 Japan
3 Department of Physiology, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Nagoya, 466-8550 Japan
4 CREST, Japan Science and Technology Agency (JST), Saitama, 332-0012 Japan
5 Department of Biology, Tokyo Gakugei University, 4-1-1 Nukuikita-machi, Koganei-shi, Tokyo, 184-8501 Japan
6 Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki, 444-858 Japan
Mechanosensitive ion channels are expected to play important roles in transducing mechanical stimuli into intracellular signals during the development and morphogenesis of higher plants. We have identified a novel mechanosensitive anion channel in the protoplast of Arabidopsis thaliana mesophyll cells by using the patch-clamp technique. The channel in the outside-out patches could be activated by positive pressure in the pipette while negative pressure had no effect. The amphipathic membrane crenator trinitrophenol, which is supposed to preferentially insert in the outer leaflet of the lipid bilayer of the plasma membrane, synergized with mechanical membrane stretch to activate the channel. These results suggest that the channel activation is mediated by a convex curvature of the plasma membrane. Therefore, activation of this channel may play an important role when cell volume is increasing during cell growth or hypo-osmotic challenge, which is accompanied by membrane stretch with increasingly convex curvature.
7 Corresponding authors: qizhi{at}sun5.ibp.ac.cn; Fax: +86-010-64853605; msokabe{at}med.nagoya-u.ac.jp; Fax: +8152-744-2057.
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