© 2004 Oxford University Press
Enhancement of Cyclic Electron Flow Around PSI at High Light and its Contribution to the Induction of Non-Photochemical Quenching of Chl Fluorescence in Intact Leaves of Tobacco Plants
Research Institute of Innovative Technology for the Earth (RITE), 9-2 Kizugawadai, Kizu-cho, Soraku-gun, Kyoto, 619-0292 Japan
Non-photochemical quenching (NPQ) of Chl fluorescence is a mechanism for dissipating excess photon energy and is dependent on the formation of a 
pH across the thylakoid membranes. The role of cyclic electron flow around photosystem I (PSI) (CEF-PSI) in the formation of this pH was elucidated by studying the relationships between O2-evolution rate [V(O2)], quantum yield of both PSII and PSI [
(PSII) and (PSI)], and Chl fluorescence parameters measured simultaneously in intact leaves of tobacco plants in CO2-saturated air. Although increases in light intensity raised V(O2) and the relative electron fluxes through both PSII and PSI [(PSII)xPFD and (PSI)xPFD] only (PSI)xPFD continued to increase after V(O2) and (PSII)xPFD became light saturated. These results revealed the activity of an electron transport reaction in PSI not related to photosynthetic linear electron flow (LEF), namely CEF-PSI. NPQ of Chl fluorescence drastically increased after (PSII)xPFD became light saturated and the values of NPQ correlated positively with the relative activity of CEF-PSI. At low temperatures, the light-saturation point of (PSII)xPFD was lower than that of (PSI)xPFD and NPQ was high. On the other hand, at high temperatures, the light-dependence curves of (PSII)xPFD and (PSI)xPFD corresponded completely and NPQ was not induced. These results indicate that limitation of LEF induced CEF-PSI, which, in turn, helped to dissipate excess photon energy by driving NPQ of Chl fluorescence.
1 Corresponding author: E-mail, cmiyake{at}rite.or.jp; Fax, +81-774-75-2320.
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