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Plant and Cell Physiology, 2004, Vol. 45, No. 1 57-67
© 2004 Oxford University Press

Characterization of Plant Circadian Rhythms by Employing Arabidopsis Cultured Cells with Bioluminescence Reporters

Norihito Nakamichi1, Shogo Ito1, Tokitaka Oyama2, Takafumi Yamashino1, Takao Kondo2 and Takeshi Mizuno1,3

1 Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya, 464-8601 Japan
2 Graduate School of Biological Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8601 Japan

Recent intensive studies have begun to shed light on the molecular mechanisms underlying the plant circadian clock in Arabidopsis thaliana. During the course of these previous studies, the most powerful technique, elegantly adopted, was a real-time bioluminescence monitoring system of circadian rhythms in intact plants carrying a luciferase (LUC) fusion transgene. We previously demonstrated that Arabidopsis cultured cells also retain an ability to generate circadian rhythms, at least partly. To further improve the cultured cell system for studies on circadian rhythms, here we adopted a bioluminescence monitoring system by establishing the cell lines carrying appropriate reporter genes, namely, CCA1::LUC and APRR1::LUC, with which CCA1 (CIRCADIAN CLOCK-ASSOCIATED1) and APRR1 (or TOC1) (ARABIDOPSIS PSEUDO-RESPONSE REGULATORS1 or TIMING OF CAB EXPRESSION1) are believed to be the components of the central oscillator. We report the results that consistently supported the view that the established cell lines, equipped with such bioluminescence reporters, might provide us with an advantageous means to characterize the plant circadian clock.

3 Corresponding author: E-mail, tmizuno{at}agr.nagoya-u.ac.jp; Fax, +81-52-789-4091.


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