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Plant and Cell Physiology, 2004, Vol. 45, No. 1 109-113
© 2004 Oxford University Press

Short Communication

An In vivo Dual-Reporter System of Cyanobacteria Using Two Railroad-Worm Luciferases with Different Color Emissions

Yohko Kitayama1, Takao Kondo1, Yoichi Nakahira1,3, Hideya Nishimura1, Yoshihiro Ohmiya2 and Tokitaka Oyama1,4

1 Department of Biological Science, Graduate School of Science, Nagoya University and Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan
2 The Special Division for Human Life Technology, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, 565-8577 Japan

Abstract

In vivo genetic reporter systems using luciferase enzymes enable the real-time monitoring of gene expression in living cells. We have challenged concurrent monitoring of two independent promoter activities within the same cells to precisely compare their characteristics in vivo. In this report, we describe a simple dual-reporter system capable of simultaneously monitoring two promoter activities in living cyanobacterial cells. Two railroad-worm luciferases catalyzing the bioluminescent emissions of different colors served as the dual reporters; each emission was successfully separated by interference filters to estimate the individual bioluminescence signals using photomultiplier tubes. Using this system, we clearly demonstrated the difference in the expression profiles between promoters in the same cells.

Footnotes

3 Present address: Faculty of Human Environment, Kyoto Prefectural University, Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, 606–8522 Japan.

4 Corresponding author: E-mail, oyama{at}biol1.bio.nagoya-u.ac.jp; Fax, +81-52-789-2963.


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