Plant and Cell Physiology, 2004, Vol. 45, No. 1 103-108
© 2004 Oxford University Press
The Function of Chloroplastic NAD(P)H Dehydrogenase in Tobacco during Chilling Stress under Low Irradiance
College of Life Sciences, Shandong Agricultural University, Tai’an, Shandong, 271018, P.R. China
The function of chloroplastic NAD(P)H dehydrogenase (NDH) was examined by comparing a tobacco transformant (
ndhB) in which the ndhB gene had been disrupted with its wild type, upon exposure to chilling temperature (4°C) under low irradiance (100 µmol m–2 s–1 PFD). During the chilling stress, the maximum photochemical efficiency of PSII (Fv/Fm) decreased markedly in both the wild type and ndhB. However, both Fv/Fm and P700+, as well as the PSII-driven electron transport rate (ETR), in ndhB were lower than that in the wild type, implying that NDH-dependent cyclic electron flow around PSI functioned to protect the photosynthetic apparatus from chilling stress under low irradiance. Under the stress, non-photochemical quenching (NPQ), particularly the fast relaxing NPQ component (qf) and the de-epoxidized ratio of the xanthophyll cycle pigments, (A+Z)/(V+A+Z), were distinguishable in ndhB from those in the wild type. The lower NPQ in ndhB might be related to an inefficient proton gradient across thylakoid membranes (pH) because of lacking an NDH-dependent cyclic electron flow around PSI at chilling temperature under low irradiance.
1 Present address: Xin-Guo Li, Crop Institute, Shandong Academy of Agricultural Sciences, Ji’nan, Shandong, 250100, P.R. China
2 Corresponding author: E-mail, qwmeng{at}sdau.edu.cn; Fax, +86-538-8226399.
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