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Plant and Cell Physiology, 2003, Vol. 44, No. 9 914-921
© 2003 Oxford University Press

Exclusion of Ribulose-1,5-bisphosphate Carboxylase/oxygenase from Chloroplasts by Specific Bodies in Naturally Senescing Leaves of Wheat

Akira Chiba1, Hiroyuki Ishida1,3, Naoko K. Nishizawa2, Amane Makino1 and Tadahiko Mae1

1 Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555 Japan
2 Department of Global Agricultural Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan

Immunocytochemical electron-microscopic observation indicated that ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) and/or its degradation products are localized in small spherical bodies having a diameter of 0.4–1.2 µm in naturally senescing leaves of wheat (Triticum aestivum L.). These Rubisco-containing bodies (RCBs) were found in the cytoplasm and in the vacuole. RCBs contained another stromal protein, chloroplastic glutamine synthetase, but not thylakoid proteins. Ultrastructural analysis suggested that RCBs had double membranes, which seemed to be derived from the chloroplast envelope, and that RCBs were further surrounded by the other membrane structures in the cytoplasm. The appearance of RCBs was the most remarkable when the amount of Rubisco started to decrease at the early phase of leaf senescence. These results suggest that RCBs might be involved in the degradation process of Rubisco outside of chloroplasts during leaf senescence.

3 Corresponding author: E-mail, hiroyuki{at}biochem.tohoku.ac.jp; Fax, +81-22-717-8765.


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