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Plant and Cell Physiology, 2003, Vol. 44, No. 8 861-867
© 2003 Oxford University Press

Short Communications

Array-Based Analysis on Tobacco Plastid Transcripts: Preparation of a Genomic Microarray Containing All Genes and All Intergenic Regions

Takahiro Nakamura1,2, Yumiko Furuhashi2, Keiko Hasegawa1,2, Hiroshi Hashimoto3, Kazufumi Watanabe3, Junichi Obokata2, Mamoru Sugita2 and Masahiro Sugiura1,4

1 Graduate School of Natural Sciences, Nagoya City University, Yamanohata, Mizuho, Nagoya, 467-8501 Japan
2 Center for Gene Research, Nagoya University, Nagoya, 464-8602 Japan
3 Hokkaido System Science Co., Ltd, Hassamu 14-1-1-34, Nishi, Sapporo, 063-0834 Japan

Abstract

The plastid genome of higher plants includes about 120 genes. We adopted genomic array technologies to the tobacco plastid genome. A microarray was constructed, consisting of 220 DNA fragments that cover the whole genome sequence. Each DNA fragment corresponds to a single known gene or an intergenic region. We evaluated reliability of this microarray by comparing the plastid RNA level in light- or dark-grown tobacco seedlings. The transcripts encoding photosynthetic subunits increased significantly in light-grown tissues as expected. Furthermore, we found unexpected signals in several intergenic regions, suggesting the existence of novel transcripts in tobacco plastids.

Footnotes

4 Corresponding author: E-mail, sugiura{at}nsc.nagoya-cu.ac.jp; Fax, +81-52-872-6021.


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