Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Papini-Terzi, F. S.
Right arrow Articles by Van Sluys, M.-A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Papini-Terzi, F. S.
Right arrow Articles by Van Sluys, M.-A.
Agricola
Right arrow Articles by Papini-Terzi, F. S.
Right arrow Articles by Van Sluys, M.-A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2003, Vol. 44, No. 8 856-860
© 2003 Oxford University Press

Short Communications

Point Mutation is Responsible for Arabidopsis tz-201 Mutant Phenotype Affecting Thiamin Biosynthesis

Flávia Stal Papini-Terzi1,3, Rodrigo da Silva Galhardo2, Leonardo Paiva Farias1,4, Carlos Frederico Martins Menck2 and Marie-Anne Van Sluys1,5

1 Depto. de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, 277-05508-900 São Paulo, SP, Brazil
2 Depto. de Microbiologia, Inst.de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374-05508-900 São Paulo, SP, Brazil

Abstract

A point mutation in the thi1 gene, involved in the synthesis of thiamin, has been identified in a tz-201 mutant line of Arabidopsis thaliana. The mutation occurs in a conserved protein domain and prevents the mutant plants from synthesizing thiamin. Complementation assays in yeast thi4 mutant confirm that this mutation hinders thiamin synthesis and, thus, is responsible for the tz phenotype. Northern blot analyses indicate that, in contrast to the yeast homologue, thi1 expression is not influenced by the presence of thiamin; however, reduced transcription of the gene is observed in roots and dark grown plants.

5 Corresponding author: E-mail, mavsluys{at}usp.br; Fax, +55-11-3091-7547.

4 Present address: Centro de Biotecnologia – Bio Molecular, Instituto Butantan, Av. Vital Brasil, 1500 - Butantã, 05503-900 São Paulo, SP, Brazil.

Footnotes

3 Present address: Depto. de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748 - sala 1208, 05508-900 São Paulo, SP, Brazil.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
Z. Deng, X. Zhang, W. Tang, J. A. Oses-Prieto, N. Suzuki, J. M. Gendron, H. Chen, S. Guan, R. J. Chalkley, T. K. Peterman, et al.
A Proteomics Study of Brassinosteroid Response in Arabidopsis
Mol. Cell. Proteomics, December 1, 2007; 6(12): 2058 - 2071.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. H. C. Godoi, R. S. Galhardo, D. D. Luche, M.-A. Van Sluys, C. F. M. Menck, and G. Oliva
Structure of the Thiazole Biosynthetic Enzyme THI1 from Arabidopsis thaliana
J. Biol. Chem., October 13, 2006; 281(41): 30957 - 30966.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
D. T. Ribeiro, L. P. Farias, J. D. de Almeida, P. M. Kashiwabara, A. F. C. Ribeiro, M. C. Silva-Filho, C. F. M. Menck, and M.-A. Van Sluys
Functional characterization of the thi1 promoter region from Arabidopsis thaliana
J. Exp. Bot., July 1, 2005; 56(417): 1797 - 1804.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.