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Plant and Cell Physiology, 2003, Vol. 44, No. 7 726-734
© 2003 Oxford University Press

Distinct Expression and Function of Three Ammonium Transporter Genes (OsAMT1;1 – 1;3) in Rice

Yutaka Sonoda1,7, Akira Ikeda1,2,7, Satomi Saiki4, Nicolaus von Wirén4, Tomoyuki Yamaya5,6 and Junji Yamaguchi1,8

1 Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku N10-W8, Sapporo, 060-0810 Japan
2 CREST, Japan Science and Technology Corporation (JST)
Division of Bioresources and Product Science, Graduate School of Agriculture, Hokkaido University, Kita-ku N9-W9, Sapporo, 060-8589 Japan
4 Institut für Pflanzenernährung, Universität Hohenheim, D-70593 Stuttgart, Germany
5 Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, 981-8555 Japan
6 Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, 230-0045 Japan

To study the regulation of ammonium uptake into rice roots, three ammonium transporter genes (OsAMT1;1, 1;2 and 1;3; Oryza sativa ammonium transporter) were isolated and examined. OsAMT1s belong to AMT1 family, containing 11 putative transmembrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1–1;3 the complete AMT1 family of rice had been isolated. Heterologous expression of OsAMT1s in the yeast Saccharomyces cerevisiae mutant 31019b showed that all three OsAMT1s exhibit ammonium transport activity. Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3. In situ mRNA detection revealed that OsAMT1;2 is expressed in the central cylinder and cell surface of root tips. This gene expression analysis revealed a distinct nitrogen-dependent regulation for AMTs in rice, differing from that in tomato or Arabidopsis.

7 Y.S. and A.I. contributed equally to this work.

8 Corresponding author: E-mail, jjyama{at}sci.hokudai.ac.jp; Fax, +81-11-706-2737.


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