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Plant and Cell Physiology, 2003, Vol. 44, No. 6 637-642
© 2003 Oxford University Press

Short Communications

Assessment of Utility of Meiosis-Associated Promoters of Lily for Induction of Germinal Ds Transposition in Transgenic Rice

Ryouhei Morita1, Yoko Hattori1, Shuji Yokoi2, Hisabumi Takase2, Masayoshi Minami2, Kazuyuki Hiratsuka3 and Kinya Toriyama1,4

1 Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555 Japan
2 Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, 630-0101 Japan
3 Division of Plant Biotechnology, Graduate School of Environment and Information Sciences, Yokohama National University, Yokohama, 240-8501 Japan

Abstract

In order to suppress the somatic excision of the Ds element and increase the independent transposition events of the Ac/Ds transposon tagging system in rice, we employed promoters of two meiosis-specific genes of lily, LIM10 and LIM18. The LIM10 promoter directed GUS expression specifically in anthers, with the LIM18 promoter doing the same in the anthers and somatic tissue. Both promoters induced independent germinal transposition with the frequency of approximately 1%. The LIM10 promoter, lacking induction of somatic transposition, is considered to be useful for improving transposon-tagging efficiencies in rice.

Footnotes

4 Corresponding author: E-mail, torikin{at}bios.tohoku.ac.jp; Fax, +81–22–717–8654.


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