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Plant and Cell Physiology, 2003, Vol. 44, No. 4 447-450
© 2003 Oxford University Press

Short Communications

Transformation of Tobacco with a Gene for the Thermophilic Acyl-Lipid Desaturase Enhances the Chilling Tolerance of Plants

Irina V. Orlova1,2, Tatiana S. Serebriiskaya1, Valeri Popov1, Natalia Merkulova1, Alexander M. Nosov1, Tamara I. Trunova1, Vladimir D. Tsydendambaev1 and Dmitry A. Los1,3

1 Institute of Plant Physiology, Russian Academy of Science, Botanicheskaya Street 35, 127276 Moscow, Russia
2 Branch of Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Science Avenue 6, 142290 Pushchino, Moscow Region, Russia

Abstract

The desC gene for the acyl-lipid {Delta}9-desaturase from the thermophilic cyanobacterium Synechococcus vulcanus was introduced into Nicotiana tabacum under control of the 35S promoter. Expression of the desaturase was confirmed by Western blotting. Lipid analysis revealed that lipid content and the extent of fatty acid unsaturation significantly increased in leaves of transgenic plants. Chilling tolerance of those plants also increased, as estimated by the electrolyte leakage from the tissues damaged by cold treatments. Seeds of plants that expressed the desC gene imbibed at low temperatures demonstrated higher chilling tolerance than those of the control plants. The results demonstrate that the cyanobacterial thermophilic acyl-lipid desaturase was efficiently expressed in tobacco at ambient temperatures, and its expression resulted in the enhanced chilling tolerance of the transgenic plants.

Footnotes

3 Corresponding author: Email, losnet{at}ippras.ru; Fax, +7-095-977-9372.


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