Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hayashi, H.
Right arrow Articles by Inoue, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hayashi, H.
Right arrow Articles by Inoue, K.
Agricola
Right arrow Articles by Hayashi, H.
Right arrow Articles by Inoue, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2003, Vol. 44, No. 4 404-411
© 2003 Oxford University Press

Up-regulation of Soyasaponin Biosynthesis by Methyl Jasmonate in Cultured Cells of Glycyrrhiza glabra

Hiroaki Hayashi1, Pengyu Huang and Kenichiro Inoue

Department of Pharmacognosy, Gifu Pharmaceutical University, Mitahora-higashi 5-6-1, Gifu, 502-8585 Japan

Exogenously applied methyl jasmonate (MeJA) stimulated soyasaponin biosynthesis in cultured cells of Glycyrrhiza glabra (common licorice). mRNA level and enzyme activity of ß-amyrin synthase (bAS), an oxidosqualene cyclase (OSC) situated at the branching point for oleanane-type triterpene saponin biosynthesis, were up-regulated by MeJA, whereas those of cycloartenol synthase, an OSC involved in sterol biosynthesis, were relatively constant. Two mRNAs of squalene synthase (SQS), an enzyme common to both triterpene and sterol biosyntheses, were also up-regulated by MeJA. In addition, enzyme activity of UDP-glucuronic acid: soyasapogenol B glucuronosyltransferase, an enzyme situated at a later step of soyasaponin biosynthesis, was also up-regulated by MeJA. Accumulations of bAS and two SQS mRNAs were not transient but lasted for 7 d after exposure to MeJA, resulting in the high-level accumulation (more than 2% of dry weight cells) of soyasaponins in cultured licorice cells. In contrast, bAS and SQS mRNAs were coordinately down-regulated by yeast extract, and mRNA accumulation of polyketide reductase, an enzyme involved in 5-deoxyflavonoid biosynthesis in cultured licorice cells, was induced transiently by yeast extract and MeJA, respectively.

1 Corresponding author: E-mail, hayashih{at}gifu-pu.ac.jp; Fax, +81-58-237-5979.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
M.-H. Lee, J.-H. Jeong, J.-W. Seo, C.-G. Shin, Y.-S. Kim, J.-G. In, D.-C. Yang, J.-S. Yi, and Y.-E. Choi
Enhanced Triterpene and Phytosterol Biosynthesis in Panax ginseng Overexpressing Squalene Synthase Gene
Plant Cell Physiol., August 15, 2004; 45(8): 976 - 984.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Zhao, S.-H. Zheng, K. Fujita, and K. Sakai
Jasmonate and ethylene signalling and their interaction are integral parts of the elicitor signalling pathway leading to {beta}-thujaplicin biosynthesis in Cupressus lusitanica cell cultures
J. Exp. Bot., May 1, 2004; 55(399): 1003 - 1012.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.