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Plant and Cell Physiology, 2003, Vol. 44, No. 4 377-387
© 2003 Oxford University Press

The cDNA Microarray Analysis Using an Arabidopsis pad3 Mutant Reveals the Expression Profiles and Classification of Genes Induced by Alternaria brassicicola Attack

Yoshihiro Narusaka1,2,5,6, Mari Narusaka3, Motoaki Seki2,3, Junko Ishida3, Maiko Nakashima3, Asako Kamiya3, Akiko Enju3, Tetsuya Sakurai3, Masakazu Satoh3, Masatomo Kobayashi4, Yukio Tosa1, Pyoyun Park1 and Kazuo Shinozaki2,3

1 Kobe University Graduate School of Science and Technology, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501 Japan
2 Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, 305-0074 Japan
3 Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045 Japan
4 Experimental Plant Division, RIKEN Bio Resource Center, 3-1-1 Koyadai, Tsukuba, 305-0074 Japan

The hypersensitive response (HR) was induced in a wild-type Arabidopsis thaliana plant (Columbia) (Col-wt) by inoculation with Alternaria brassicicola that causes the development of small brown necrotic lesions on the leaves. By contrast, pad3-1 mutants challenged with A. brassicicola produced spreading lesions. The cell death in pad3-1 mutants could not inhibit the pathogen growth and development, although both production of H2O2 and localized cell death were similar in Col-wt and pad3-1 plants after the inoculation. The difference between Col-wt and pad3-1 plants is defense responses after the occurrence of cell death. In other words, PAD3 is necessary for defense response to A. brassicicola. Therefore, we examined the changes in the expression patterns of ca. 7,000 genes by cDNA microarray analysis after inoculation with A. brassicicola. The cDNA microarrays were also done to analyze Arabidopsis responses after treatment with signal molecules, reactive oxygen species (ROS)-inducing compounds and UV-C. The results suggested that the pad3-1 mutation altered not only the accumulation of camalexin but also the timing of expression of many defense-related genes in response to the challenge with A. brassicicola. Furthermore, the plants integrate two or more signals that act together for promoting the induction of multiple defense pathways.

5 The first two authors contributed equally to this work.

6 Corresponding author: E-mail, narusaka{at}ans.ans.kobe-u.ac.jp; Fax, +81-78-803-6540.


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