Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Takahashi, H.
Right arrow Articles by Inoue, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takahashi, H.
Right arrow Articles by Inoue, Y.
Agricola
Right arrow Articles by Takahashi, H.
Right arrow Articles by Inoue, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2003, Vol. 44, No. 3 350-359
© 2003 Oxford University Press

Randomization of Cortical Microtubules in Root Epidermal Cells Induces Root Hair Initiation in Lettuce (Lactuca sativa L.) Seedlings

Hidenori Takahashi1, Kayoko Hirota, Aiko Kawahara, Erika Hayakawa and Yasunori Inoue

Department of Applied Biological Sciences, Faculty of Science and Technology, Tokyo University of Science, Yamazaki 2641, Noda, Chiba, 278-8510 Japan

Root hair formation is induced when lettuce seedlings are transferred from liquid medium at pH 6.0 to fresh medium at pH 4.0. If seedlings are transferred to pH 6.0, no root hairs are formed. We investigated the role of microtubules in this low pH-induced root hair initiation in lettuce. At the hair-forming zone in root epidermal cells, microtubules were perpendicular to the longitudinal axis of the cell just after pre-culture. This arrangement became disordered as early as 5 min after transfer to pH 4.0, and became random by 30 min later. At pH 4.0, the randomization extended to the entire hair-forming zone of seedlings; at pH 6.0, however, randomization did not occur and transverse microtubules were maintained. When seedlings at pH 6.0 were treated with microtubule-depolymerizing drugs, root hairs were formed. In contrast, when a microtubule-stabilizing drug, taxol, was added to the medium, no root hairs formed, even at pH 4.0. These results suggest that the transverse cortical microtubules inhibit root hair formation, and that their destruction is necessary for initiation. Furthermore, the microfilament-disrupting drugs cytochalasin B and latrunculin B inhibited root hair initiation, suggesting that actin filaments are necessary for root hair initiation.

1 Corresponding author: E-mail, takahide{at}rs.noda.tus.ac.jp; Fax, +81-4-7123-9767.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
N. Kanzawa, Y. Hoshino, M. Chiba, D. Hoshino, H. Kobayashi, N. Kamasawa, Y. Kishi, M. Osumi, M. Sameshima, and T. Tsuchiya
Change in the Actin Cytoskeleton during Seismonastic Movement of Mimosa pudica
Plant Cell Physiol., April 1, 2006; 47(4): 531 - 539.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Van Bruaene, G. Joss, and P. Van Oostveldt
Reorganization and in Vivo Dynamics of Microtubules during Arabidopsis Root Hair Development
Plant Physiology, December 1, 2004; 136(4): 3905 - 3919.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. Takahashi, A. Kawahara, and Y. Inoue
Ethylene Promotes the Induction by Auxin of the Cortical Microtubule Randomization Required for Low-pH-Induced Root Hair Initiation in Lettuce (Lactuca sativa L.) Seedlings
Plant Cell Physiol., September 15, 2003; 44(9): 932 - 940.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.