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Plant and Cell Physiology, 2003, Vol. 44, No. 3 296-303
© 2003 Oxford University Press

C-Terminal Domain of a Hevein-Like Protein from Wasabia japonica has Potent Antimicrobial Activity

Akinori Kiba1,2,3, Hiromasa Saitoh1, Masahiro Nishihara1, Kaori Omiya1 and Saburo Yamamura1

1 Iwate Biotechnology Research Center, Kitakami, Iwate, 024-0003 Japan
2 Present address: Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University, Nankoku, 783-8502 Japan

An antimicrobial protein, designated WjAMP-1, was purified from leaves of Wasabia japonica L. WjAMP-1 showed antimicrobial activity against both fungi and bacteria. The deduced amino acid sequence of cDNA of WjAMP-1 showed 60% and 70% identity with a hevein from Hevea brasiliansis and a hevein-like protein from Arabidopsis thaliana, respectively. However, matured WjAMP-1 lacked the hevein domain and may correspond to the C-terminal domain of hevein. Southern blot analysis showed that one or two copies of the WjAMP-1 gene were presented in the genome of wasabi. Expression of WjAMP-1 was detected in all organs tested, and was especially strong in petioles. Expression of WjAMP-1 was induced by the inoculation with fungal pathogens and treatment with methyl jasmonate. Recombinant WjAMP-1 expressed in Nicotiana benthamiana using potato virus X vector also inhibited not only growth of fungi but also bacteria. These results suggest that WjAMP-1 may be the C-terminal domain of hevein and one of the defense gene in W. japonica. WjAMP-1 gene may be useful genes to generate resistant plants against fungal and bacterial pathogens.

3 Corresponding author: E-mail, akiba{at}cc.kochi-u.ac.jp; Fax, +81-88-864-5219.


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