Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Yoshida, K.
Right arrow Articles by Kondo, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshida, K.
Right arrow Articles by Kondo, T.
Agricola
Right arrow Articles by Yoshida, K.
Right arrow Articles by Kondo, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2003, Vol. 44, No. 3 262-268
© 2003 Oxford University Press

Sepal Color Variation of Hydrangea macrophylla and Vacuolar pH Measured with a Proton-Selective Microelectrode

Kumi Yoshida1,4, Yuki Toyama-Kato2, Kiyoshi Kameda2 and Tadao Kondo3

1 Graduate School of Human Informatics, Nagoya University, Chikusa, Nagoya, 464-8601 Japan
2 School of Life Studies, Sugiyama Jogakuen University, Chikusa, Nagoya, 464-8662 Japan
3 Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601 Japan

Sepal color of hydrangea varies with the environmental conditions. Although chemical and biological studies on this color variation have a long history, little correct knowledge has been generated about color development. All colored sepals contain the same anthocyanin, delphinidin 3-glucoside. Thus, there must be some other system for developing the wide variety of colors. In hydrangea sepals the cells of the epidermis are colorless and only the second layer of cells contain pigment. We prepared protoplasts without any color change during enzyme treatment of sepals and measured the vacuolar pH of each of the colored cells. We could correlate the color of a single hydrangea cell with its vacuolar pH using a combination of micro-spectrophotometry and a proton-selective microelectrode. Values for the vacuolar pH of blue ({lambda}vismax: 589 nm) and red cells ({lambda}vismax: 537 nm) were 4.1 and 3.3, respectively, the vacuolar pH of blue cells being significantly higher.

4 Corresponding author: E-mail, yoshidak{at}info.human.nagoya-u.ac.jp; Fax, +81-52-789-5638.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
Y. Katsumoto, M. Fukuchi-Mizutani, Y. Fukui, F. Brugliera, T. A. Holton, M. Karan, N. Nakamura, K. Yonekura-Sakakibara, J. Togami, A. Pigeaire, et al.
Engineering of the Rose Flavonoid Biosynthetic Pathway Successfully Generated Blue-Hued Flowers Accumulating Delphinidin
Plant Cell Physiol., November 1, 2007; 48(11): 1589 - 1600.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Shoji, N. Miki, N. Nakajima, K. Momonoi, C. Kato, and K. Yoshida
Perianth Bottom-Specific Blue Color Development in Tulip cv. Murasakizuisho Requires Ferric Ions
Plant Cell Physiol., February 1, 2007; 48(2): 243 - 251.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. Quattrocchio, W. Verweij, A. Kroon, C. Spelt, J. Mol, and R. Koes
PH4 of Petunia Is an R2R3 MYB Protein That Activates Vacuolar Acidification through Interactions with Basic-Helix-Loop-Helix Transcription Factors of the Anthocyanin Pathway
PLANT CELL, May 1, 2006; 18(5): 1274 - 1291.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Yoshida, M. Kawachi, M. Mori, M. Maeshima, M. Kondo, M. Nishimura, and T. Kondo
The Involvement of Tonoplast Proton Pumps and Na+(K+)/H+ Exchangers in the Change of Petal Color During Flower Opening of Morning Glory, Ipomoea tricolor cv. Heavenly Blue
Plant Cell Physiol., March 1, 2005; 46(3): 407 - 415.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.