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Plant and Cell Physiology, 2003, Vol. 44, No. 2 132-138
© 2003 Oxford University Press

Changes in {alpha}-Xylosidase during Intact and Auxin-Induced Growth of Pine Hypocotyls

María Sánchez, Cristina Gianzo, Javier Sampedro, Gloria Revilla and Ignacio Zarra1

Departamento de Fisiología Vegetal, Facultad de Biología, Universidad de Santiago de Compostela, E-15782 Santiago de Compostela, Spain

A complete cDNA from Pinus pinaster Aiton, potentially coding for an {alpha}-xylosidase able to remove the xylose residue from xyloglucan oligosaccharides, has been cloned. Its sequence was homologous to previously published {alpha}-xylosidase genes from Arabidopsis and nasturtium. The protein also showed the two signature regions of family 31 of glycosyl hydrolases. The gene expression level was quantified by competitive RT-PCR, under different growth conditions, throughout seedling development, in different regions along the hypocotyls and in auxin-treated hypocotyl segments, and related with growth capacity and {alpha}-xylosidase activity. A role of {alpha}-xylosidase in regulating the level of xyloglucan oligosaccharides within the apoplast is proposed. The action of an {alpha}-xylosidase removing the xylose residue, would make possible the action of a ß-glucosidase deblocking the xyloglucan oligosaccharide degradation and it could serve as a control point for the regulation of the apoplastic levels of xyloglucan oligosaccharides.

1 Corresponding author: E-mail: bvzarra{at}usc.es; Fax: 34-981-596904.


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