Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Oh, M.-H.
Right arrow Articles by Lee, C.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oh, M.-H.
Right arrow Articles by Lee, C.-H.
Agricola
Right arrow Articles by Oh, M.-H.
Right arrow Articles by Lee, C.-H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2003, Vol. 44, No. 12 1368-1377
© 2003 Oxford University Press

Increased Stability of LHCII by Aggregate Formation during Dark-Induced Leaf Senescence in the Arabidopsis Mutant, ore10

Min-Hyuk Oh, Yong-Hwan Moon and Choon-Hwan Lee1

Department of Molecular Biology, Pusan National University, Busan, 609-735 Korea

Leaf senescence in a stay-green mutant of Arabidopsis thaliana, ore10, was investigated during dark-incubation of its detached leaves. During this dark-induced senescence (DIS), Chl loss was delayed in ore10 mutants, as compared with wild type, but the rate of decline in the photochemical efficiency of PSII was not delayed in mutant leaves. After 2 d of DIS, native green gel electrophoresis of ore 10 leaf proteins resulted in a significant amount of pigment remaining as aggregates on top of the stacking gel. In addition, the accumulation of aggregates coincided with the emergence of a new band near 700 nm (F699) in the 77 K fluorescence emission spectrum of the aggregates. At 4 d, F699 became a major band, both in the isolated aggregates and in intact leaves. Prolonged treatment with detergents revealed that light-harvesting complex II (LHCII) remaining after 2 d was highly stable, and the accumulation of aggregates coincided with the appearance of truncated LHCII in senescing ore10 leaves. These results suggest that increased LHCII stability is due to the formation of aggregates of trimmed LHCII. Thus, the LHCII protein degradation step that follows proteolysis of its terminal peptides is a possible lesion site of the ore10 mutant.

1 Corresponding author: E-mail, chlee{at}pusan.ac.kr; Fax, +82-51-513-9258.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
S. Schelbert, S. Aubry, B. Burla, B. Agne, F. Kessler, K. Krupinska, and S. Hortensteiner
Pheophytin Pheophorbide Hydrolase (Pheophytinase) Is Involved in Chlorophyll Breakdown during Leaf Senescence in Arabidopsis
PLANT CELL, March 1, 2009; 21(3): 767 - 785.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Alos, M. Roca, D. J. Iglesias, M. I. Minguez-Mosquera, C. M. B. Damasceno, T. W. Thannhauser, J. K. C. Rose, M. Talon, and M. Cercos
An Evaluation of the Basis and Consequences of a Stay-Green Mutation in the navel negra Citrus Mutant Using Transcriptomic and Proteomic Profiling and Metabolite Analysis
Plant Physiology, July 1, 2008; 147(3): 1300 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Ren, K. An, Y. Liao, X. Zhou, Y. Cao, H. Zhao, X. Ge, and B. Kuai
Identification of a Novel Chloroplast Protein AtNYE1 Regulating Chlorophyll Degradation during Leaf Senescence in Arabidopsis
Plant Physiology, July 1, 2007; 144(3): 1429 - 1441.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S.-Y. Park, J.-W. Yu, J.-S. Park, J. Li, S.-C. Yoo, N.-Y. Lee, S.-K. Lee, S.-W. Jeong, H. S. Seo, H.-J. Koh, et al.
The Senescence-Induced Staygreen Protein Regulates Chlorophyll Degradation
PLANT CELL, May 1, 2007; 19(5): 1649 - 1664.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Kusaba, H. Ito, R. Morita, S. Iida, Y. Sato, M. Fujimoto, S. Kawasaki, R. Tanaka, H. Hirochika, M. Nishimura, et al.
Rice NON-YELLOW COLORING1 Is Involved in Light-Harvesting Complex II and Grana Degradation during Leaf Senescence
PLANT CELL, April 1, 2007; 19(4): 1362 - 1375.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F.-Q. Guo and N. M. Crawford
Arabidopsis Nitric Oxide Synthase1 Is Targeted to Mitochondria and Protects against Oxidative Damage and Dark-Induced Senescence
PLANT CELL, December 1, 2005; 17(12): 3436 - 3450.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.