Plant and Cell Physiology, 2003, Vol. 44, No. 11 1168-1175
© 2003 Oxford University Press
A New 9-Lipoxygenase cDNA from Developing Rice Seeds
1 Institute of Agricultural and Forest Engineering, University of Tsukuba, Tsukuba Science City, Ibaraki, 305-8572 Japan
2 Material Science Research Center, Shiseido Co. Ltd., Yokohama, 224-8558 Japan
We isolated a novel C9 position specific lipoxygenase (r9-LOX1) cDNA from developing rice seeds. The enzymatic features of r9-LOX1 resembled those of rice LOX-L3 known to be contained in rice germ and to have C9-specific LOX activity. However, the expression level of the r9-LOX1 gene was higher in imbibed seeds rather than developing seeds. A homology search against the rice nucleotide database revealed the r9-LOX1 gene to be on rice chromosome 3 (accession number AC093017). The restriction enzyme map of the reported genomic sequence agreed with the result of the Southern blot analysis for the r9-LOX1. The enzyme could be useful for in vitro synthesis of 9,10-ketol-octadecadienoic acid.
3 Present address: Faculty of Bioresource Sciences, Akita Prefectural University, Akita City, Akita, 010-0195 Japan.
4 Corresponding author: E-mail, koumno{at}akita-pu.ac.jp; Fax, +81-18-872-1677.
![]()
CiteULike
Connotea
Del.icio.us What's this?
This article has been cited by other articles:
![]() |
A. Nemchenko, S. Kunze, I. Feussner, and M. Kolomiets Duplicate maize 13-lipoxygenase genes are differentially regulated by circadian rhythm, cold stress, wounding, pathogen infection, and hormonal treatments J. Exp. Bot., November 1, 2006; 57(14): 3767 - 3779. [Abstract] [Full Text] [PDF] |
||||
