Plant and Cell Physiology, 2003, Vol. 44, No. 10 1081-1087
© 2003 Oxford University Press
Mucilage Secretion Regulated by Sex Pheromones in Closterium peracerosum-strigosum-littorale Complex
1 Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Ora-gun, Gunma, 374-0193 Japan
2 Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902 Japan
3 Institute of Biological Sciences, University of Tsukuba, Ibaraki, 305-8572 Japan
4 Faculty of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Ora-gun, Gunma, 374-0193 Japan
When mating-type plus (mt+) and minus (mt–) cells of the Closterium peracerosum-strigosum-littorale complex were mixed in nitrogen-depleted mating medium, secretion of mucilage containing uronic acid from cells was markedly activated and the mucilage accumulated around the cells. Substances with the ability to stimulate mucilage secretion from mt+ and mt– cells were detected in media in which mt– and mt+ cells had been separately cultured, respectively. We designated the active substances secreted from mt+ and mt– cells mucilage secretion-stimulating pheromone (MS-SP)-plus and MS-SP-minus, respectively. Activity of MS-SP-plus and MS-SP-minus decreased to 20% level by incubation at 80°C for 10 min. Light was indispensable for the secretion of mucilage. The secretion of MS-SP-plus and MS-SP-minus decreased with dark treatment. MS-SP-plus eluted at around 95 k from a gel filtration column, and reacted with antibodies against two subunits of protoplast-release-inducing protein (PR-IP), which induces protoplast release from mt– cells. MS-SP-minus eluted at around 20 k from a gel filtration column, and reacted with an antibody against the PR-IP inducer, which induces the secretion of PR-IP from mt+ cells. In addition, purified PR-IP and PR-IP inducer stimulated mucilage secretion from mt– and mt+ cells, respectively. These results strongly suggested that MS-SP-plus and MS-SP-minus were the same molecules as the PR-IP and the PR-IP inducer, respectively.
5 Present address: Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture & Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588 Japan.
6 Corresponding author: E-mail, sekimoto{at}bio.c.u-tokyo.ac.jp; Fax, +81-3-5454-6644.
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