Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kakizaki, T.
Right arrow Articles by Watanabe, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kakizaki, T.
Right arrow Articles by Watanabe, M.
Agricola
Right arrow Articles by Kakizaki, T.
Right arrow Articles by Watanabe, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2003, Vol. 44, No. 1 70-75
© 2003 Oxford University Press

Linear Dominance Relationship among Four Class-II S Haplotypes in Pollen is Determined by the Expression of SP11 in Brassica Self-Incompatibility

Tomohiro Kakizaki1, Yoshinobu Takada1, Akiko Ito1,4, Go Suzuki2, Hiroshi Shiba3, Seiji Takayama3, Akira Isogai3 and Masao Watanabe1,5

1 Faculty of Agriculture, Iwate University, Morioka, 020-8550 Japan
2 Division of Natural Science, Osaka Kyoiku University, Kashiwara, 582-8582 Japan
3 Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0101 Japan

Self-incompatibility (SI) prevents self-fertilization by rejecting pollen from plants with the same S phenotype. The Brassica SI system is controlled sporophytically by multiple alleles at the single locus, S, and dominance relationships among S haplotypes are observed in both stigma and pollen. We have identified previously five different class-II S haplotypes in Brassica campestris. Here, we performed test-crosses between S heterozygotes and their respective parental S homozygotes for four of these class-II S haplotypes, and observed a linear dominance relationship on the pollen side. To determine how this relationship is controlled, we performed RNA gel blot analyses for six S heterozygotes and their respective parental S homozygotes using the corresponding SP11 clone as a probe. In all six S heterozygotes, SP11 derived from a dominant haplotype was predominantly expressed, and SP11 derived from a recessive haplotype was repressed. Thus, the linear dominance relationship of the SI phenotype on the pollen side is regulated by the expression of SP11.

4 Current address: Iwate Biotechnology Research Center, Kitakami, 024-0003 Japan.

5 Corresponding author: E-mail, nabe@iwate-u.ac.jp; Fax, +81-(0)19-621-6177.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
N. L. Prigoda, A. Nassuth, and B. K. Mable
Phenotypic and Genotypic Expression of Self-incompatibility Haplotypes in Arabidopsis lyrata Suggests Unique Origin of Alleles in Different Dominance Classes
Mol. Biol. Evol., July 1, 2005; 22(7): 1609 - 1620.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.