Plant and Cell Physiology, 2003, Vol. 44, No. 1 25-34
© 2003 Oxford University Press
Photoperiodic Regulation of a 24-kD Dehydrin-Like Protein in Red-Osier Dogwood (Cornus sericea L.) in Relation to Freeze-Tolerance
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907-1165, U.S.A.
A predominant 24-kD dehydrin-like protein was previously found to fluctuate seasonally within red-osier dogwood (Cornus sericea L.) stems. The current study attempted to determine what environmental cues triggered the accumulation of the 24-kD protein and to assess its potential role in winter survival. Controlled photoperiod and field studies confirmed that photoperiod regulates a reduction of stem water content (SWC), freeze-tolerance enhancement and accumulation of the 24-kD protein. Diverse climatic ecotypes, which are known to respond to different critical photoperiods, displayed differential reduction of SWC and accumulation of the 24-kD protein. A time-course study confirmed that prolonged exposure to short days is essential for SWC reduction, 24-kD protein accumulation, and freeze-tolerance enhancement. Water deficit induced 24-kD protein accumulation and enhanced freeze-tolerance under long-day conditions. In all instances, freeze-tolerance enhancement and 24-kD protein accumulation was preceded by a reduction of SWC. These results are consistent with the hypothesis that C. sericea responds to decreasing photoperiod, which triggers a reduction in SWC. Reduced SWC in turn may trigger the accumulation of the 24-kD protein and a parallel increase in freeze-tolerance.
1 Present address of Dale T. Karlson is Department of Low Temperature Sciences, National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Sapporo, 062855 Japan.
2 Present address of Yan Zeng is Veteran’s Administration Medical Center, Indianapolis, IN 46202, U.S.A.
3 Corresponding author: E-mail, ashworth@hort.purdue.edu; Fax, +1-765-494-0391.
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