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Plant and Cell Physiology, 2002, Vol. 43, No. 9 1054-1058
© 2002 Oxford University Press

Short Communications

Salicylic Acid Carboxyl Methyltransferase Induced in Hairy Root Cultures of Atropa belladonna after Treatment with Exogeneously Added Salicylic Acid

Hiroyuki Fukami1, Tomiko Asakura2, Hiroshi Hirano1, Keiko Abe3, Koichiro Shimomura4,5 and Takashi Yamakawa1,6

1 Laboratory of Plant Biotechnology, Department of Global Agricultural Sciences, The University of Tokyo, Tokyo, 113-8657 Japan
2 Laboratory of Food Science, Atomi Junior College, Tokyo, 112-8687 Japan
3 Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657 Japan
4 Tsukuba Medicinal Plant Research Station, National Institute of Health Sciences, Tsukuba, 305-0843 Japan

Abstract

In Atropa belladonna hairy roots, exogeneously added salicylic acid (SA) is converted to methyl salicylate (MSA) through the reaction, which might be catalysed by S-adenosyl-L-methionine: salicylic acid carboxyl methyltransferase (SAMT). Here we cloned a cDNA for A. belladonna SAMT (AbSAMT1), which consisted of 357 aa residues. It was expressed in E. coli, and the recombinant AbSAMT1 showed SAMT activity. When A. belladonna hairy roots were exposed to a high concentration of SA, AbSAMT1 mRNA begins to be expressed 12 h after the exposure, and steady expression continued over 144 h.

Footnotes

5 Present address: Faculty of Life Sciences, Toyo University, Itakura, Gunma, 374-0193 Japan.

6 Corresponding author: E-mail, ayama@mail.ecc.u-tokyo.ac.jp; Fax, +81-3-5841-5304.


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