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Plant and Cell Physiology, 2002, Vol. 43, No. 7 810-815
© 2002 Oxford University Press

Spatial Expression and Characterization of a Putative Ethylene Receptor Protein NTHK1 in Tobacco

Can Xie1, Zhi-Gang Zhang, Jin-Song Zhang2, Xin-Jian He, Wan-Hong Cao, Si-Jie He and Shou-Yi Chen2

Plant Biotechnology Lab, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, PR China

A putative ethylene receptor gene NTHK1 encodes a protein with a putative signal peptide, three transmembrane segments, a putative histidine kinase domain and a putative receiver domain. The receiver domain was expressed in an Escherichia coli expression system, purified and used to generate polyclonal antibodies for immunohistochemistry analysis. The spatial expression of the NTHK1 protein was then investigated. We found that NTHK1 was abundant during flower and ovule development. It was also expressed in glandular hairs, stem, and in leaves that had been wounded. The NTHK1 gene was further introduced into the tobacco plant and we found that, in different transgenic lines, the NTHK1 gene was transcribed to various degrees. Upon ACC treatment, the etiolated transgenic seedlings showed reduced ethylene sensitivity when compared with the control, indicating that NTHK1 is a functional ethylene receptor in plants.

1 Present address: Center for Blood Research, Harvard Medical School.

2 Corresponding author: E-mail, sychen@genetics.ac.cn; jszhang@genetics.ac.cn; Fax, +86-10-6487-3428.


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