Plant and Cell Physiology, 2002, Vol. 43, No. 7 751-758
© 2002 Oxford University Press
An Increase in Unsaturation of Fatty Acids in Phosphatidylglycerol from Leaves Improves the Rates of Photosynthesis and Growth at Low Temperatures in Transgenic Rice Seedlings
1 Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, 981-8555 Japan
2 Laboratory of Plant Ecology, Graduate School of Science, Tokyo University, Bunkyo-Ku, 7-3-1 Hongo, Tokyo, 113-0033 Japan
3 Department of Regulation Biology, National Institute for Basic Biology, Okazaki, 444-0867 Japan
The level of cis-unsaturated fatty acids in phosphatidylglycerol (PG) from rice leaves was genetically altered from 19.3% in the wild-type to 29.4 and 32.0% in T1 plants segregated with cDNAs for glycerol-3-phosphate acyltransferase of chloroplasts (GPAT; EC 2.3.1.15) from Arabidopsis (+AGPAT plant) and spinach (+SGPAT plant), respectively; and to 21.4% in a non-transformant segregated from +SGPAT plants (–SGPAT plant). In all these plants, O2 evolution from leaves was similar at 25°C and was impaired to a similar extent at 5 and 11°C. However, in parallel with the levels of cis-unsaturated fatty acids in PG, +AGPAT and +SGPAT plants showed less impaired rates of O2 evolution from leaves than the wild-type and –SGPAT plants at 14 and 17°C. In agreement with this, the fresh weight of 14-day-old seedlings increased to 571±18, 591±23, 687±32 and 705±31 mg in the wild-type, –SGPAT, +AGPAT and +SGPAT plants, respectively, after 6 weeks at 17/14°C (day/night). These results demonstrate the practical importance of the present technology with GPAT in improvement of the chilling sensitivity of crops.
4 Corresponding author: E-mail, torikin@bios.tohoku.ac.jp; Fax, +81-22-717-8654.
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