Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (41)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Li, Y.-B.
Right arrow Articles by Jiang, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, Y.-B.
Right arrow Articles by Jiang, L.
Agricola
Right arrow Articles by Li, Y.-B.
Right arrow Articles by Jiang, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2002, Vol. 43, No. 7 726-742
© 2002 Oxford University Press

BP-80 and Homologs are Concentrated on Post-Golgi, Probable Lytic Prevacuolar Compartments

Yu-Bing Li1, Sally W. Rogers2, Yu Chung Tse1, Sze Wan Lo1, Samuel S. M. Sun1, Guang-Yuh Jauh3 and Liwen Jiang1,4

1 Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
2 Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, U.S.A.
3 Institute of Botany, Academic Sinica, Taiwan

Prevacuolar compartments (PVCs) are membrane-bound organelles that mediate protein traffic between Golgi and vacuoles in the plant secretory pathway. Here we identify and define organelles as the lytic prevacuolar compartments in pea and tobacco cells using confocal immunofluorescence. We use five different antibodies specific for a vacuolar sorting receptor (VSR) BP-80 and its homologs to detect the location of VSR proteins. In addition, we use well-established Golgi-markers to identify Golgi organelles. We further compare VSR-labeled organelles to Golgi organelles so that the relative proportion of VSR proteins in Golgi vs. PVCs can be quantitated. More than 90% of the BP-80-marked organelles are separate from Golgi organelles; thus, BP-80 and its homologs are predominantly concentrated on the lytic PVCs. Additionally, organelles marked by anti-AtPep12p (AtSYP21p) and anti-AtELP antibodies are also largely separate from Golgi apparatus, whereas VSR and AtPep12p (AtSYP21p) were largely colocalized. We have thus demonstrated in plant cells that VSR proteins are predominantly present in the lytic PVCs and have provided additional markers for defining plant PVCs using confocal immunofluorescence. Additionally, our approach will provide a rapid comparison between markers to quantitate protein distribution among various organelles.

4 Corresponding author: E-mail, ljiang@cuhk.edu.hk; Fax, +852-2603-5646.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
E. Limpens, S. Ivanov, W. van Esse, G. Voets, E. Fedorova, and T. Bisseling
Medicago N2-Fixing Symbiosomes Acquire the Endocytic Identity Marker Rab7 but Delay the Acquisition of Vacuolar Identity
PLANT CELL, September 1, 2009; 21(9): 2811 - 2828.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Wang, Y. Cai, Y. Miao, S. K. Lam, and L. Jiang
Wortmannin induces homotypic fusion of plant prevacuolar compartments
J. Exp. Bot., July 1, 2009; 60(11): 3075 - 3083.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. Hwang
Sorting and Anterograde Trafficking at the Golgi Apparatus
Plant Physiology, October 1, 2008; 148(2): 673 - 683.
[Full Text] [PDF]

Home page
 J Exp BotHome page
E. Onelli, C. Prescianotto-Baschong, M. Caccianiga, and A. Moscatelli
Clathrin-dependent and independent endocytic pathways in tobacco protoplasts revealed by labelling with charged nanogold
J. Exp. Bot., August 1, 2008; 59(11): 3051 - 3068.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. G. Robinson, L. Jiang, and K. Schumacher
The Endosomal System of Plants: Charting New and Familiar Territories
Plant Physiology, August 1, 2008; 147(4): 1482 - 1492.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. K. Lam, Y. Cai, S. Hillmer, D. G. Robinson, and L. Jiang
SCAMPs Highlight the Developing Cell Plate during Cytokinesis in Tobacco BY-2 Cells
Plant Physiology, August 1, 2008; 147(4): 1637 - 1645.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. L. Avila, M. Brown, S. Pan, R. Desikan, S. J. Neill, T. Girke, M. Surpin, and N. V. Raikhel
Expression analysis of Arabidopsis vacuolar sorting receptor 3 reveals a putative function in guard cells
J. Exp. Bot., April 1, 2008; 59(6): 1149 - 1161.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Muller, U. Mettbach, D. Menzel, and J. Samaj
Molecular Dissection of Endosomal Compartments in Plants
Plant Physiology, October 1, 2007; 145(2): 293 - 304.
[Full Text] [PDF]

Home page
 J Exp BotHome page
K. Muntz
Protein dynamics and proteolysis in plant vacuoles
J. Exp. Bot., July 1, 2007; 58(10): 2391 - 2407.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Wang, Y. Li, S. W. Lo, S. Hillmer, S. S.M. Sun, D. G. Robinson, and L. Jiang
Protein Mobilization in Germinating Mung Bean Seeds Involves Vacuolar Sorting Receptors and Multivesicular Bodies
Plant Physiology, April 1, 2007; 143(4): 1628 - 1639.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Fuji, T. Shimada, H. Takahashi, K. Tamura, Y. Koumoto, S. Utsumi, K. Nishizawa, N. Maruyama, and I. Hara-Nishimura
Arabidopsis Vacuolar Sorting Mutants (green fluorescent seed) Can Be Identified Efficiently by Secretion of Vacuole-Targeted Green Fluorescent Protein in Their Seeds
PLANT CELL, February 1, 2007; 19(2): 597 - 609.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. K. Lam, C. L. Siu, S. Hillmer, S. Jang, G. An, D. G. Robinson, and L. Jiang
Rice SCAMP1 Defines Clathrin-Coated, trans-Golgi-Located Tubular-Vesicular Structures as an Early Endosome in Tobacco BY-2 Cells
PLANT CELL, January 1, 2007; 19(1): 296 - 319.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. C. Tse, S. W. Lo, S. Hillmer, P. Dupree, and L. Jiang
Dynamic Response of Prevacuolar Compartments to Brefeldin A in Plant Cells
Plant Physiology, December 1, 2006; 142(4): 1442 - 1459.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Miao, P. K. Yan, H. Kim, I. Hwang, and L. Jiang
Localization of Green Fluorescent Protein Fusions with the Seven Arabidopsis Vacuolar Sorting Receptors to Prevacuolar Compartments in Tobacco BY-2 Cells
Plant Physiology, November 1, 2006; 142(3): 945 - 962.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. S. Otegui, R. Herder, J. Schulze, R. Jung, and L. A. Staehelin
The Proteolytic Processing of Seed Storage Proteins in Arabidopsis Embryo Cells Starts in the Multivesicular Bodies
PLANT CELL, October 1, 2006; 18(10): 2567 - 2581.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
O. Foresti, L. L.P. daSilva, and J. Denecke
Overexpression of the Arabidopsis Syntaxin PEP12/SYP21 Inhibits Transport from the Prevacuolar Compartment to the Lytic Vacuole in Vivo
PLANT CELL, September 1, 2006; 18(9): 2275 - 2293.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. L.P. daSilva, O. Foresti, and J. Denecke
Targeting of the Plant Vacuolar Sorting Receptor BP80 Is Dependent on Multiple Sorting Signals in the Cytosolic Tail
PLANT CELL, June 1, 2006; 18(6): 1477 - 1497.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Oliviusson, O. Heinzerling, S. Hillmer, G. Hinz, Y. C. Tse, L. Jiang, and D. G. Robinson
Plant Retromer, Localized to the Prevacuolar Compartment and Microvesicles in Arabidopsis, May Interact with Vacuolar Sorting Receptors
PLANT CELL, May 1, 2006; 18(5): 1239 - 1252.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Oufattole, J. H. Park, M. Poxleitner, L. Jiang, and J. C. Rogers
Selective Membrane Protein Internalization Accompanies Movement from the Endoplasmic Reticulum to the Protein Storage Vacuole Pathway in Arabidopsis
PLANT CELL, November 1, 2005; 17(11): 3066 - 3080.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. U. Kim, Y. Li, and A. H.C. Huang
Ubiquitous and Endoplasmic Reticulum-Located Lysophosphatidyl Acyltransferase, LPAT2, Is Essential for Female but Not Male Gametophyte Development in Arabidopsis
PLANT CELL, April 1, 2005; 17(4): 1073 - 1089.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Kim, M. Park, S. J. Kim, and I. Hwang
Actin Filaments Play a Critical Role in Vacuolar Trafficking at the Golgi Complex in Plant Cells
PLANT CELL, March 1, 2005; 17(3): 888 - 902.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. L.P. daSilva, J. P. Taylor, J. L. Hadlington, S. L. Hanton, C. J. Snowden, S. J. Fox, O. Foresti, F. Brandizzi, and J. Denecke
Receptor Salvage from the Prevacuolar Compartment Is Essential for Efficient Vacuolar Protein Targeting
PLANT CELL, January 1, 2005; 17(1): 132 - 148.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. M. Kotzer, F. Brandizzi, U. Neumann, N. Paris, I. Moore, and C. Hawes
AtRabF2b (Ara7) acts on the vacuolar trafficking pathway in tobacco leaf epidermal cells
J. Cell Sci., December 15, 2004; 117(26): 6377 - 6389.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G.-J. Lee, E. J. Sohn, M. H. Lee, and I. Hwang
The Arabidopsis Rab5 Homologs Rha1 and Ara7 Localize to the Prevacuolar Compartment
Plant Cell Physiol., September 15, 2004; 45(9): 1211 - 1220.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. C. Tse, B. Mo, S. Hillmer, M. Zhao, S. W. Lo, D. G. Robinson, and L. Jiang
Identification of Multivesicular Bodies as Prevacuolar Compartments in Nicotiana tabacum BY-2 Cells
PLANT CELL, March 1, 2004; 16(3): 672 - 693.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Bolte, S. Brown, and B. Satiat-Jeunemaitre
The N-myristoylated Rab-GTPase m-Rabmc is involved in post-Golgi trafficking events to the lytic vacuole in plant cells
J. Cell Sci., February 22, 2004; 117(6): 943 - 954.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
U. NEUMANN, F. BRANDIZZI, and C. HAWES
Protein Transport in Plant Cells: In and Out of the Golgi{dagger}
Ann. Bot., August 1, 2003; 92(2): 167 - 180.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. J. Sohn, E. S. Kim, M. Zhao, S. J. Kim, H. Kim, Y.-W. Kim, Y. J. Lee, S. Hillmer, U. Sohn, L. Jiang, et al.
Rha1, an Arabidopsis Rab5 Homolog, Plays a Critical Role in the Vacuolar Trafficking of Soluble Cargo Proteins
PLANT CELL, May 1, 2003; 15(5): 1057 - 1070.
[Abstract] [Full Text]

Home page
 Plant CellHome page
P. Pimpl, S. L. Hanton, J. P. Taylor, L. L. Pinto-daSilva, and J. Denecke
The GTPase ARF1p Controls the Sequence-Specific Vacuolar Sorting Route to the Lytic Vacuole
PLANT CELL, May 1, 2003; 15(5): 1242 - 1256.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.