Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Arai, Y.
Right arrow Articles by Yamaguchi, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arai, Y.
Right arrow Articles by Yamaguchi, I.
Agricola
Right arrow Articles by Arai, Y.
Right arrow Articles by Yamaguchi, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2002, Vol. 43, No. 5 555-562
© 2002 Oxford University Press

Synthesis of a Novel Class of Polyhydroxyalkanoates in Arabidopsis Peroxisomes, and Their Use in Monitoring Short-Chain-Length Intermediates of ß-Oxidation

Yuko Arai1,2, Hideo Nakashita1,3, Yoshikatu Suzuki1, Yumiko Kobayashi1, Toshiyuki Shimizu1, Michiko Yasuda1,2, Yoshiharu Doi1 and Isamu Yamaguchi1,2

1 RIKEN Institute, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198 Japan 2 Graduate School of Science and Engineering, Saitama University, 255 Shimookubo, Saitama-shi, Saitama, 338-8570 Japan

The poly[(R)-3-hydroxyalkanoate] (PHA) synthase gene (phaCAc) of Aeromonas caviae FA440 was modified by adding a peroxisome targeting signal encoding the last 10 amino acids at the carboxyl-terminal of spinach glycolate oxidase. The modified gene was introduced into Arabidopsis thaliana plants by Agrobacterium-mediated transformation. The transgenic Arabidopsis plant expressed the introduced gene and its protein, and it accumulated PHA in its tissues. Gas chromatography-mass spectrometry analysis demonstrated the accumulation of a novel type of PHA, poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate). This strongly suggests that short-chain-length (R)-3-hydroxyacyl-CoAs were generated from intermediates of peroxisomal ß-oxidation. It was revealed by using this transgenic plant that Tween-20 can activate peroxisomal ß-oxidation of short-chain-length fatty acids.

3 Corresponding author: E-mail, nakashi@postman.riken.go.jp; Fax, +81-48-462-4959.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
Y. Arai, T. Shikanai, Y. Doi, S. Yoshida, I. Yamaguchi, and H. Nakashita
Production of Polyhydroxybutyrate by Polycistronic Expression of Bacterial Genes in Tobacco Plastid
Plant Cell Physiol., September 15, 2004; 45(9): 1176 - 1184.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Lin, J. E. Cluette-Brown, and H. M. Goodman
The Peroxisome Deficient Arabidopsis Mutant sse1 Exhibits Impaired Fatty Acid Synthesis
Plant Physiology, June 1, 2004; 135(2): 814 - 827.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.