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Plant and Cell Physiology, 2002, Vol. 43, No. 5 555-562
© 2002 Oxford University Press

Synthesis of a Novel Class of Polyhydroxyalkanoates in Arabidopsis Peroxisomes, and Their Use in Monitoring Short-Chain-Length Intermediates of ß-Oxidation

Yuko Arai1,2, Hideo Nakashita1,3, Yoshikatu Suzuki1, Yumiko Kobayashi1, Toshiyuki Shimizu1, Michiko Yasuda1,2, Yoshiharu Doi1 and Isamu Yamaguchi1,2

1 RIKEN Institute, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198 Japan 2 Graduate School of Science and Engineering, Saitama University, 255 Shimookubo, Saitama-shi, Saitama, 338-8570 Japan

The poly[(R)-3-hydroxyalkanoate] (PHA) synthase gene (phaCAc) of Aeromonas caviae FA440 was modified by adding a peroxisome targeting signal encoding the last 10 amino acids at the carboxyl-terminal of spinach glycolate oxidase. The modified gene was introduced into Arabidopsis thaliana plants by Agrobacterium-mediated transformation. The transgenic Arabidopsis plant expressed the introduced gene and its protein, and it accumulated PHA in its tissues. Gas chromatography-mass spectrometry analysis demonstrated the accumulation of a novel type of PHA, poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate). This strongly suggests that short-chain-length (R)-3-hydroxyacyl-CoAs were generated from intermediates of peroxisomal ß-oxidation. It was revealed by using this transgenic plant that Tween-20 can activate peroxisomal ß-oxidation of short-chain-length fatty acids.

3 Corresponding author: E-mail, nakashi@postman.riken.go.jp; Fax, +81-48-462-4959.


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