Plant and Cell Physiology, 2002, Vol. 43, No. 2 230-238
© 2002 Oxford University Press
Short Communications |
Characterization of Tobacco MADS-box Genes Involved in Floral Initiation
Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, 790-784 Korea
Abstract
MADS-box genes encode regulatory factors that are involved at various stages in plant development. These genes function not only during early floral meristem identity, but also when the fate of floral organ primordia is determined in a later step. Here, we screened a floral bud cDNA library to isolate a tobacco MADS-box gene, NtMADS4, using the rice MADS-box gene, OsMADS1, as a probe. We previously reported that OsMADS1 plays a critical role in flower development in rice. Ectopic expression of NtMADS4 caused phenotypes of extremely early flowering as well as dwarfism. Plant MADS proteins have a K domain that mediates the formation of dimers. This dimerization appears to be an essential step for a functional protein complex. NtMADS11 was isolated as an interacting partner of NtMADS4 by yeast two-hybrid screening. The latter was included in the AGAMOUS-like 2 (AGL2) family whereas the former was categorized in the SQUAMOSA (SQUA) family. While the transcript of NtMADS4 was detectable only in reproductive organs, that of NtMADS11 was seen in both reproductive and vegetative organs. Expression levels were high for both genes during early developmental stages. Ectopic expression of NtMADS11 and OsMADS14 was able to rescue the floral organ defects seen in the strong ap1-1 mutant. Roles of NtMADS4 and NtMADS11 in the floral initiation are discussed.
Footnotes
1 Corresponding author: E-mail, genean@postech.ac.kr; Fax, +82-54-279-2199.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y.-J. Liu, A. Nunes-Nesi, S. V. Wallstrom, I. Lager, A. M. Michalecka, F. E.B. Norberg, S. Widell, K. M. Fredlund, A. R. Fernie, and A. G. Rasmusson A Redox-Mediated Modulation of Stem Bolting in Transgenic Nicotiana sylvestris Differentially Expressing the External Mitochondrial NADPH Dehydrogenase Plant Physiology, July 1, 2009; 150(3): 1248 - 1259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. N. Danilevskaya, X. Meng, D. A. Selinger, S. Deschamps, P. Hermon, G. Vansant, R. Gupta, E. V. Ananiev, and M. G. Muszynski Involvement of the MADS-Box Gene ZMM4 in Floral Induction and Inflorescence Development in Maize Plant Physiology, August 1, 2008; 147(4): 2054 - 2069. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-K. Chen, I-C. Lin, and C.-H. Yang Functional Analysis of Three Lily (Lilium longiflorum) APETALA1-like MADS Box Genes in Regulating Floral Transition and Formation Plant Cell Physiol., May 1, 2008; 49(5): 704 - 717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Dong, T. M. Burch-Smith, Y. Liu, P. Mamillapalli, and S. P. Dinesh-Kumar A Ligation-Independent Cloning Tobacco Rattle Virus Vector for High-Throughput Virus-Induced Gene Silencing Identifies Roles for NbMADS4-1 and -2 in Floral Development Plant Physiology, December 1, 2007; 145(4): 1161 - 1170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Adam, F. Ouellet, N. A. Kane, Z. Agharbaoui, G. Major, Y. Tominaga, and F. Sarhan Overexpression of TaVRN1 in Arabidopsis Promotes Early Flowering and Alters Development Plant Cell Physiol., August 1, 2007; 48(8): 1192 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gallego-Giraldo, J. L. Garcia-Martinez, T. Moritz, and I. Lopez-Diaz Flowering in Tobacco Needs Gibberellins but is not Promoted by the Levels of Active GA1 and GA4 in the Apical Shoot Plant Cell Physiol., June 1, 2007; 48(6): 897 - 898. [Full Text] [PDF]
|
|
|
|
|
|
L. Gallego-Giraldo, J. L. Garcia-Martinez, T. Moritz, and I. Lopez-Diaz Flowering in Tobacco Needs Gibberellins but is not Promoted by the Levels of Active GA1 and GA4 in the Apical Shoot Plant Cell Physiol., April 1, 2007; 48(4): 615 - 625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Fornara, L. Parenicova, G. Falasca, N. Pelucchi, S. Masiero, S. Ciannamea, Z. Lopez-Dee, M. M. Altamura, L. Colombo, and M. M. Kater Functional Characterization of OsMADS18, a Member of the AP1/SQUA Subfamily of MADS Box Genes Plant Physiology, August 1, 2004; 135(4): 2207 - 2219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Litt and V. F. Irish Duplication and Diversification in the APETALA1/FRUITFULL Floral Homeotic Gene Lineage: Implications for the Evolution of Floral Development Genetics, October 1, 2003; 165(2): 821 - 833. [Abstract] [Full Text] [PDF]
|
|