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Plant and Cell Physiology, 2002, Vol. 43, No. 12 1510-1517
© 2002 Oxford University Press

Utilization and Transport of Glucose in Olea Europaea Cell Suspensions

Jorge Oliveira, Rui M. Tavares and Hernâni Gerós1

Centro de Ciências do Ambiente, Department of Biology, University of Minho, 4710-057 Braga, Portugal

Cell suspensions of Olea europaea var. Galega Vulgar grown in batch culture with 0.5% (w/v) glucose were able to transport D-[14C]glucose according to Michaelis–Menten kinetics associated with a first-order kinetics. The monosaccharide carrier exhibited high affinity (Km {approx}50 µM) and was able to transport D-glucose, D-fructose, D-galactose, D-xylose, 2-deoxy-D-glucose and 3-O-methyl-D-glucose, but not D-arabinose, D-mannitol or L-glucose. D-[14C]glucose uptake was associated with proton uptake, which also followed Michaelis–Menten kinetics. The transport of 3-O-methyl-D-glucose was accumulative (40-fold, at pH 5.0) and the protonophore carbonyl cyanide m-chlorophenylhydrazone strongly inhibited sugar accumulation. The results were consistent with the involvement of a monosaccharide: proton symporter with a stoichiometry of 1 : 1. When cells were grown with 3% (w/v) glucose, the uptake of D-[14C]glucose followed first-order kinetics and monosaccharide:proton symporter activity was not detected. The value obtained for the permeability coefficient of hexoses in O. europaea cells supported the hypothesis that the first-order kinetics observed in 0.5% and 3% sugar-grown cells was produced exclusively by passive diffusion of the sugar. The results indicate that in O. europaea cells sugar levels have a regulatory effect on sugar transport, because the activity for monosaccharide transport was repressed by high sugar concentrations.

1 Corresponding author: E-mail, geros@bio.uminho.pt; Fax, + 351-253-678980.


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